Abstract
Eco-friendly biosynthetic approach for silver nanoparticles production using plant extracts is an exciting advancement in bio-nanotechnology and has been successfully attempted in nearly 41 plant species. However, an established model plant system for systematically unraveling the biochemical components required for silver nanoparticles production is lacking. Here we used Arabidopsis thaliana as the model plant for silver nanoparticles biosynthesis in vitro. Employing biochemical, spectroscopic methods, selected mutants and over-expressor plants of Arabidopsis involved in pleotropic functions and sugar homeostasis, we show that carbohydrates, polyphenolics and glyco-proteins are essential components which stimulated silver nanoparticles synthesis. Using molecular genetics as a tool, our data enforces the requirement of sugar conjugated proteins as essentials for AgNPs synthesis over protein alone. Additionally, a comparative analysis of AgNPs synthesis using the aqueous extracts of some of the plant species found in a brackish water ecosystem (Gracilaria, Potamogeton, Enteromorpha and Scendesmus) were explored. Plant extract of Potamogeton showed the highest potential of nanoparticles production comparable to that of Arabidopsis among the species tested. Silver nanoparticles production in the model plant Arabidopsis not only opens up a possibility of using molecular genetics tool to understand the biochemical pathways and components in detail for its synthesis.
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Acknowledgements
This work was supported by Department of Biotechnology, Ministry of Science and Technology, Grant Number: BT/PR15236/BRB/10/916/2011. We thank Department of Atomic Energy, India, and NISER for the experimental facility offered at the institute. We also would like to thank Prof. PV Satyam from the Institute of Physics, Bhubaneswar, for their help in conducting the FESEM.
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Kumar, A., Kumar, A.A., Nayak, A.P. et al. Carbohydrates and polyphenolics of extracts from genetically altered plant acts as catalysts for in vitro synthesis of silver nanoparticle. J Biosci 44, 6 (2019). https://doi.org/10.1007/s12038-018-9826-6
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DOI: https://doi.org/10.1007/s12038-018-9826-6