Skip to main content

Advertisement

SpringerLink
Log in

Protein-rich extract of Musca domestica larvae alleviated metabolic disorder in STZ-induced type 2 diabetic rat model via hepatoprotective and pancreatic β-cell protective activities

  • Published:
Journal of Biosciences Aims and scope Submit manuscript

A Correction to this article was published on 24 October 2019

This article has been updated

Abstract

The study was designed to explore the beneficial effect of Musca domestica larvae extract (MDLE) on a metabolic disorder using a diabetic rat model. Streptozotocin-induced diabetic rats were treated with or without MDLE. Blood glucose, insulin levels, lipid profiles, and oxidative stress markers were measured. The morphological changes in the pancreas and liver were determined, as well as insulin expression. The expression of glucose transporter 4 (GLUT4), phospho-adenosine monophosphate-activated protein kinase (p-AMPK)/total AMPK, superoxide dismutase 1 (SOD1), catalase (CAT), and peroxisome proliferator-activated receptor gamma (PPARγ) were detected. Compared with untreated diabetic rats, MDLE-treated rats had decreased urine volume, food intake, and water intake, along with significantly lower levels of blood glucose, malondialdehyde (MDA), plasma triglycerides, low-density lipoprotein (LDL), and total cholesterol. MDLE-treated rats also had higher levels of SOD activity, high-density lipoprotein (HDL), and insulin. MDLE treatment partially restored the β-cell population, improved the liver necrosis and islet cell damage, reversed the decreased expression of GLUT4, phospho-AMPK, SOD1, and CAT in the liver, skeletal muscle and pancreatic tissue, and also increased the expression of PPARγ in the liver and adipose tissue in diabetic rats. In conclusion, the obtained results suggest that MDLE could possibly be used pharmacologically as an adjuvant for the treatment of diabetes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7

Similar content being viewed by others

Change history

  • 24 October 2019

    In the December 2018 issue of the Journal of Biosciences, in the article titled ‘‘Protein-rich extract of Musca domestica larvae alleviated metabolic disorder in STZ-induced type 2 diabetic rat model via hepatoprotective and pancreatic β -cell protective activities’’ by Hanfang Mei et al. (DOI: 10.1007/s12038-018-9804-z; Vol. 43, No. 5, pp. 969–983), the affiliations of Hanfang Mei have been incompletely mentioned as:

References

  • Ai H, Wang F, Zhang N, Zhang L and Lei C 2013 Antiviral, immunomodulatory, and free radical scavenging activities of a protein-enriched fraction from the larvae of the housefly, Musca domestica. J. Insect. Sci. 13 112

    PubMed  Google Scholar 

  • Baynes JW 1991 Role of oxidative stress in development of complications in diabetes. Diabetes 40 405–412

    Article  CAS  Google Scholar 

  • Bilen MA, Pan T, Lee YC, Lin SC, Yu G, Pan J, Hawke D, Pan BF, Vykoukal J, Gray K, Satcher RL, Gallick GE, Yu-Lee LY and Lin SH 2017 Proteomics profiling of exosomes from primary mouse osteoblasts under proliferation versus mineralization conditions and characterization of their uptake into prostate cancer cells. J Proteome Res. 16 2709–2728

    Article  CAS  Google Scholar 

  • Chen F, Xiong H, Wang J, Ding X, Shu G and Mei Z 2013 Antidiabetic effect of total flavonoids from Sanguis draxonis in type 2 diabetic rats. J. Ethnopharmacol. 149 729–736

    Article  CAS  Google Scholar 

  • Chu FJ, Jin XB, Xu YY, Ma Y, Li XB, Lu XM, Liu WB and Zhu JY 2013 Inflammatory regulation effect and action mechanism of anti-inflammatory effective parts of housefly (Musca domestica) larvae on atherosclerosis. Evid. Based Complement Alternat. Med. 2013 340267

    PubMed  PubMed Central  Google Scholar 

  • Chu FJ, Jin XB and Zhu JY 2011 Housefly maggots (Musca domestica) protein-enriched fraction/extracts (PE) inhibit lipopolysaccharide-induced atherosclerosis pro-inflammatory responses. J. Atheroscler. Thromb. 18 282–290

    Article  CAS  Google Scholar 

  • Fu P, Wu J and Guo G 2009 Purification and molecular identification of an antifungal peptide from the hemolymph of Musca domestica (housefly). Cell. Mol. Immunol. 6 245–51

    Article  CAS  Google Scholar 

  • Gastaldelli A, Miyazaki Y, Pettiti M, Matsuda M, Mahankali S, Santini E, Defronzo RA and Ferrannini E 2002 Metabolic effects of visceral fat accumulation in type 2 diabetes. J. Clin. Endocrinol. Metab. 87 5098–5103

    Article  CAS  Google Scholar 

  • Guo S 2014 Insulin signaling, resistance, and the metabolic syndrome: insights from mouse models into disease mechanisms. J. Endocrinol. 220 T1–T23

    Article  CAS  Google Scholar 

  • He K, Li X, Chen X, Ye X, Huang J, Jin Y, Li P, Deng Y, Jin Q, Shi Q and Shu H 2011 Evaluation of antidiabetic potential of selected traditional Chinese medicines in STZ-induced diabetic mice. J. Ethnopharmacol. 137 1135–1142

    Article  Google Scholar 

  • Hou L, Shi Y, Zhai P and Le G 2007 Antibacterial activity and in vitro anti-tumor activity of the extract of the larvae of the housefly (Musca domestica). J. Ethnopharmacol. 111 227–231

    Article  Google Scholar 

  • Ishihama Y, Oda Y, Tabata T, Sato T, Nagasu T, Rappsilber J and Mann M 2005 Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein. Mol. Cell. Proteomics 4 1265–1272

    Article  CAS  Google Scholar 

  • Jin XB, Mei HF, Pu QH, Shen J, Lu XM, Chu FJ and Zhu JY 2013 Effects of Musca domestica cecropin on the adhesion and migration of human hepatocellular carcinoma BEL-7402 cells. Biol. Pharm. Bull. 36 938–943

    Article  CAS  Google Scholar 

  • Jing YJ, Hao YJ, Qu H, Shan Y, Li DS and Du RQ 2007 Studies on the antibacterial activities and mechanisms of chitosan obtained from cuticles of housefly larvae. Acta Biol. Hung. 58 75–86

    Article  CAS  Google Scholar 

  • Kayama Y, Raaz U, Jagger A, Adam M, Schellinger IN, Sakamoto M, Suzuki H, Toyama K, Spin JM and Tsao PS 2015 Diabetic Cardiovascular Disease Induced by Oxidative Stress. Int. J. Mol. Sci. 16 25234–25263

    Article  CAS  Google Scholar 

  • Keane KN, Cruzat VF, Carlessi R, De Bittencourt PI, Jr. and Newsholme P 2015 Molecular events linking oxidative stress and inflammation to insulin resistance and beta-cell dysfunction. Oxid. Med. Cell Longev. 2015 181643

    Article  Google Scholar 

  • Kersten S 2002 Peroxisome proliferator activated receptors and obesity. Eur. J. Pharmacol. 440 223–234

    Article  CAS  Google Scholar 

  • Kowluru RA and Mishra M 2015 Oxidative stress, mitochondrial damage and diabetic retinopathy. Biochim. Biophys. Acta 1852 2474–2483

    Article  CAS  Google Scholar 

  • Li XM 2007 Protective effect of Lycium barbarum polysaccharides on streptozotocin-induced oxidative stress in rats. Int. J. Biol. Macromol. 40 461–465

    Article  CAS  Google Scholar 

  • Li YX, Jin XB, Chu FJ, Liu MY, Shi DY and Zhu JY 2013 Scientific evaluation of the subchronic toxicity of Musca domestica larvae extracts in Sprague Dawley rats. Food Chem. Toxicol. 59 464–469

    Article  CAS  Google Scholar 

  • Liu M, Wang Y, Liu Y and Ruan R 2016 Bioactive peptides derived from traditional Chinese medicine and traditional Chinese food: A review. Food Res. Int. 89 63–73

    Article  CAS  Google Scholar 

  • Mai TT, Yamaguchi K, Yamanaka M, Lam NT, Otsuka Y and Chuyen NV 2010 Protective and anticataract effects of the aqueous extract of Cleistocalyx operculatus flower buds on beta-cells of streptozotocin-diabetic rats. J. Agric. Food Chem. 58 4162–4168

    Article  CAS  Google Scholar 

  • Martinez De Toda I and De La Fuente M 2015 The role of Hsp70 in oxi-inflamm-aging and its use as a potential biomarker of lifespan. Biogerontology 16 709–721

    Article  CAS  Google Scholar 

  • Ogunji JO, Nimptsch J, Wiegand C and Schulz C 2007 Evaluation of the influence of housefly maggot meal (magmeal) diets on catalase, glutathione S-transferase and glycogen concentration in the liver of Oreochromis niloticus fingerling. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 147 942–947

    Article  Google Scholar 

  • Ren GD and Shi AM 2002 Factory production and its developmental future for house flies. Chinese Bull. Entomol. 10 103

    Google Scholar 

  • Shih CC, Lin CH, Lin WL and Wu JB 2009 Momordica charantia extract on insulin resistance and the skeletal muscle GLUT4 protein in fructose-fed rats. J. Ethnopharmacol. 123 82–90

    Article  Google Scholar 

  • Wang FR, Ai H, Chen XM and Lei CL 2007 Hepatoprotective effect of a protein-enriched fraction from the maggots (Musca domestica) against CCl4-induced hepatic damage in rats. Biotechnol. Lett. 29 853–858

    Article  CAS  Google Scholar 

  • Zhang B, Kang M, Xie Q, Xu B, Sun C, Chen K and Wu Y 2011 Anthocyanins from Chinese bayberry extract protect beta cells from oxidative stress-mediated injury via HO-1 upregulation. J. Agric. Food Chem. 59 537–545

    Article  CAS  Google Scholar 

  • Zhi H, Ou B, Xiao XY, Peng YL, Wang Y, Liu LS, Xiao Y, Liu SJ, Wu CJ, Jiang YX, Parajuly SS, Xu P, Hao Y, Li J and Luo BM 2013 Ultrasound elastography of breast lesions in chinese women: a multicenter study in China. Clin. Breast Cancer 13 392–400

    Article  CAS  Google Scholar 

  • Zhu L, Wang P, Qin QL, Zhang H and Wu YJ 2013 Protective effect of polypeptides from larva of housefly (Musca domestica) on hydrogen peroxide-induced oxidative damage in HepG2 cells. Food Chem. Toxicol. 60 385–390

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The present study was supported by grants from the National Natural Science Foundation of China (NSFC, 81401150; NSFC, 81403129); and the Science and Technology Cooperation Research Project of Chinese Medicinal Medicine Industry, Jianhe, Guizhou, China (Bureau of Science and Technology of Jianhe County No. 5).

Author information

Author notes

    Authors and Affiliations

    1. Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou, 510006, Guangdong, China

      Hanfang Mei, Wenbin Liu & Jiayong Zhu

    2. School of Basic Courses, Guangzhou, 510006, Guangdong, China

      Hanfang Mei, Yinru He, Xia Yang, Wenbin Liu & Wei Tian

    3. Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, Guangdong, China

      Hanfang Mei

    4. Department of Laboratory Science, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China

      Jianhua Xu

    5. Key Laboratory of Digital Quality Evaluation of Chinese Material Medica of ASTCM, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, Guangdong, China

      Yu Zeng

    Authors
    1. Hanfang Mei
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Jianhua Xu
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Yinru He
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Xia Yang
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Wenbin Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Wei Tian
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Yu Zeng
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Jiayong Zhu
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding authors

    Correspondence to Yu Zeng or Jiayong Zhu.

    Additional information

    Communicated by Ullas Kolthur-Seetharam.

    Corresponding editor: Ullas Kolthur-Seetharam

    Electronic supplementary material

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Mei, H., Xu, J., He, Y. et al. Protein-rich extract of Musca domestica larvae alleviated metabolic disorder in STZ-induced type 2 diabetic rat model via hepatoprotective and pancreatic β-cell protective activities. J Biosci 43, 969–983 (2018). https://doi.org/10.1007/s12038-018-9804-z

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s12038-018-9804-z

    Keywords

    Search

    Navigation