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Fluorescence techniques in developmental biology

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Abstract

Advanced fluorescence techniques, commonly known as the F-techniques, measure the kinetics and the interactions of biomolecules with high sensitivity and spatiotemporal resolution. Applications of the F-techniques, which were initially limited to cells, were further extended to study in vivo protein organization and dynamics in whole organisms. The integration of F-techniques with multi-photon microscopy and light-sheet microscopy widened their applications in the field of developmental biology. It became possible to penetrate the thick tissues of living organisms and obtain good signal-to-noise ratio with reduced photo-induced toxicity. In this review, we discuss the principle and the applications of the three most commonly used F-techniques in developmental biology: Fluorescence Recovery After Photo-bleaching (FRAP), Förster Resonance Energy Transfer (FRET), and Fluorescence Correlation and Cross-Correlation Spectroscopy (FCS and FCCS).

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Acknowledgements

SV is supported by a Research Scholarship by the National University of Singapore. TW gratefully acknowledges funding by a grant from the Singapore Ministry of Education (MOE2016-T3-1-005).

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Authors and Affiliations

  1. Department of Biological Sciences and NUS Centre for Bio-Imaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117557, Singapore

    Sapthaswaran Veerapathiran & Thorsten Wohland

  2. Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore

    Thorsten Wohland

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  1. Sapthaswaran Veerapathiran
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  2. Thorsten Wohland
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Correspondence to Thorsten Wohland.

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Veerapathiran, S., Wohland, T. Fluorescence techniques in developmental biology. J Biosci 43, 541–553 (2018). https://doi.org/10.1007/s12038-018-9768-z

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  • DOI: https://doi.org/10.1007/s12038-018-9768-z

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