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Rice MYC2 (OsMYC2) modulates light-dependent seedling phenotype, disease defence but not ABA signalling

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Abstract

Arabidopsis MYC2 (AtMYC2) is a bHLH class transcription factor that mediates light-dependent seedling development, disease defence, JA and ABA signalling. AtMYC2 gene modulates hypocotyl elongation and expression of chlorophyll A/B binding protein 1 (CAB1) and rubisco small subunit protein1 (RBCS1) under blue light. The atmyc2 mutants are resistant against virulent bacterial pathogens. MYC2 orthologues from several crop plants have been characterized. The rice gene Os10g42430 has been referred earlier as OsMYC2 and has been shown to promote expression of JA-inducible genes. However, the role of OsMYC2 in seedling development under ABA, dark or light of specific wavelengths was not known. It was also not known whether OsMYC2 complements AtMYC2 function in Arabidopsis. We show here that expression of OsMYC2 in the atmyc2 mutant of Arabidopsis complements the blue-light-mediated defects in hypocotyl elongation and expression of CAB1 and RBCS1. We generated multiple transgenic rice lines for over-expression and RNAi-mediated suppression of OsMYC2. In agreement with AtMYC2 function, OsMYC2 over-expression and RNAi lines showed enhanced and suppressed seedling growth compared to WT plants respectively under blue light, and showed little effect under white light or dark. In agreement with the negative regulatory role of AtMYC2 in disease defence, the RNAi lines showed enhanced resistance against bacterial pathogen Xanthomonas oryzae pv oryzae. However, in contrast to AtMYC2 function, OsMYC2 influences seedling development under red light and show no effect in ABA-mediated seed germination. Thus, the results suggest evolutionarily conserved as well as the distinct role of OsMYC2 in comparison with AtMYC2.

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Acknowledgements

We acknowledge Kang Chong, Institute of Botany, Chinese Academy of Science, Beijing, for providing pTCK303 vector. We acknowledge DST-purse, Capacity Build-up and UGC resource network funds to AKN, CSIR fellowship to JKG and ICMR fellowship to MKG. Funding was provided by Department of Biotechnology, Ministry of Science and Technology (IN) (Grant No. BT/PR10678/AGR/36/581/2008).

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Author notes

  1. Mrunmay Kumar Giri and Janesh Kumar Gautam have contributed equally to this work

Authors and Affiliations

  1. School of life Sciences, Jawaharlal Nehru University, New Delhi, 110 067, India

    Mrunmay Kumar Giri, Janesh Kumar Gautam & Ashis Kumar Nandi

  2. Department of Biotechnology, National Institute of Technology, Durgapur, 713 209, India

    V. Babu Rajendra Prasad & Sudip Chattopadhyay

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  1. Mrunmay Kumar Giri
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  2. Janesh Kumar Gautam
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  3. V. Babu Rajendra Prasad
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  4. Sudip Chattopadhyay
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Correspondence to Ashis Kumar Nandi.

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Corresponding editor: Jitendra Khurana

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Giri, M.K., Gautam, J.K., Babu Rajendra Prasad, V. et al. Rice MYC2 (OsMYC2) modulates light-dependent seedling phenotype, disease defence but not ABA signalling. J Biosci 42, 501–508 (2017). https://doi.org/10.1007/s12038-017-9703-8

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