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Quaternary ammonium salt N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride induced alterations in Saccharomyces cerevisiae physiology

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Abstract:

We investigated the influence of the quaternary ammonium salt (QAS) called IM (N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride) on yeast cells of the parental strain and the IM-resistant mutant (EO25 IMR) growth. The phenotype of this mutant was pleiotropic. The IMR mutant exhibited resistance to ethanol, osmotic shock and oxidative stress, as well as increased sensitivity to UV. Moreover, it was noted that mutant EO25 appears to have an increased resistance to clotrimazole, ketoconazole, fluconazole, nystatin and cycloheximide. It also tolerated growth in the presence of crystal violet, DTT and metals (selenium, tin, arsenic). It was shown that the presence of IM decreased ergosterol level in mutant plasma membrane and increased its unsaturation. These results indicate changes in the cell lipid composition. Western blot analysis showed the induction of Pma1 level by IM. RT-PCR revealed an increased PMA1 expression after IM treatment.

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Acknowledgements

The work was supported by the Polish Ministry of Science and Higher Education grant No N N303 068 534.

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Authors and Affiliations

  1. Institute of Genetics and Microbiology, and Institute of Experimental Biology, University of Wrocław, Wrocław, Poland

    Ewa Obłąk, Agata Piecuch, Ewa Maciaszczyk-Dziubińska & Donata Wawrzycka

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  1. Ewa Obłąk
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  2. Agata Piecuch
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  3. Ewa Maciaszczyk-Dziubińska
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  4. Donata Wawrzycka
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Correspondence to Ewa Obłąk.

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Corresponding editor: Rupinder Kaur

[Obłąk E, Piecuch A, Maciaszczyk-Dziubińska E and Wawrzycka D 2016 Quaternary ammonium salt N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride induced alterations in Saccharomyces cerevisiae physiology. J. Biosci.]

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Obłąk, E., Piecuch, A., Maciaszczyk-Dziubińska, E. et al. Quaternary ammonium salt N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride induced alterations in Saccharomyces cerevisiae physiology. J Biosci 41, 601–614 (2016). https://doi.org/10.1007/s12038-016-9644-7

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