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Accelerated processing of solitary and clustered abasic site DNA damage lesions by APE1 in the presence of aqueous extract of Ganoderma lucidum

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Abstract

The stimulatory effect of the aqueous extract of G. lucidum, a basidiomycetes class fungus in the APE1-enzyme-mediated processing of solitary and bistranded clustered abasic sites DNA damages is presented. Abasic sites are considered the most common type of DNA damage lesions. Our study shows enhanced activity of APE1 in the processing of abasic sites in the presence of the polysaccharides fraction of G. lucidum. Remarkable increase in the amount of single-strand breaks (SSBs) and double-strand breaks (DSBs) from solitary and bistranded clustered abasic sites respectively with APE1 in the presence of the extract was found. This trend is maintained when abasic sites in DNA oligomers are exposed to fibroblast cell extracts in the presence of the extract. While DNA conformational alteration is negligible, APE1 enzyme shows characteristic changes in the alpha helix and beta strand ratio after incubation with G. lucidum extract. The enhanced reactivity of APE1 at the molecular level in the presence of G. lucidium is attributed to this effect. This study potentially amplifies the scope of the use of G. lucidum, which was earlier shown to have only reactive oxygen species (ROS) scavenging properties with regards to DNA damage inhibition.

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Acknowledgements

This work was supported by a grant from Department of Science and Technology, India (SERB, grant no SR/FT/LS-36/2010) to PD. BK is thankful to IIT Patna for fellowship. The author gratefully acknowledges the availability of Infrastructural and experimental facilities provided by IIT Patna. The author declares no conflict of interest. The author also acknowledges IIT BHU for analytical facilities and NIPER, Hajipur, for the gift of cultured fibroblast cells.

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Authors and Affiliations

  1. Department of Chemistry, IIT Patna, Patna, India

    Bhavini Kumari & Prolay Das

  2. Department of Zoology, A. N. College, Patna, India

    Rekha Kumari

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  1. Bhavini Kumari
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  2. Prolay Das
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  3. Rekha Kumari
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Correspondence to Prolay Das or Rekha Kumari.

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[Kumari B, Das P and Kumari R 2016 Accelerated processing of solitary and clustered abasic site DNA damage lesions by APE1 in the presence of aqueous extract of Ganoderma lucidum. J. Biosci.] DOI 10.1007/s12038-016-9614-0

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Kumari, B., Das, P. & Kumari, R. Accelerated processing of solitary and clustered abasic site DNA damage lesions by APE1 in the presence of aqueous extract of Ganoderma lucidum . J Biosci 41, 265–275 (2016). https://doi.org/10.1007/s12038-016-9614-0

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