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Signatures of positive selection at hemopexin (PEX) domain of matrix metalloproteinase-9 (MMP-9) gene

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Abstract

Matrix metalloproteinases-9 (MMP-9) is an important cancer-associated, zinc-dependent endopeptidase. To investigate the natural selection hypothesis of MMP-9, the orthologous sequences from 12 vertebrates were compared and a molecular evolution analysis was performed. Results suggest that amino acid residues present in the middle region of the protein are more selectively constrained, whereas amino acid residues in the C-terminal region of the MMP-9 protein including exon 13 showed lowest conservation level in non-primate species, suggesting that it is an exon with fast evolving rate compared to the others analyzed. InterProScan analysis shows that exon 13 was located in hemopexin (PEX) domain of MMP-9. Positive selection was detected in PEX domain of MMP-9 protein between human and other species, which indicates that selective pressure may play a role in shaping the function of MMP-9 in the course of evolution.

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Acknowledgements

This work was sponsored by National Natural Science Foundation of China (grant no. 31301486) and Natural Science Foundation of Shanghai (grant no. 13ZR1439600).

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Authors and Affiliations

  1. Shanghai Institute of Quality Inspection and Technical Research/National Quality Supervision and Inspection Center for Food Products (Shanghai), Shanghai, 200233, People’s Republic of China

    Yang Liu

  2. Department of Microbiology and Microbial Engineering, School of Life Science, Fudan University, Shanghai, 200433, People’s Republic of China

    Yang Zhao, Maobin Fu & Tonghai Dou

  3. Department of Laparoscopic Surgery, Shandong University, Linyi People’s Hospital, Linyi, Shandong Province, People’s Republic of China

    Chunlei Lu

  4. Department of Respiratory Disease, The Southern Division of Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 201112, People’s Republic of China

    Xiaoming Tan

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  1. Yang Liu
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  2. Yang Zhao
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  3. Chunlei Lu
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  4. Maobin Fu
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  5. Tonghai Dou
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Corresponding authors

Correspondence to Tonghai Dou or Xiaoming Tan.

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Corresponding editor: Stuart A. Newman

[Liu Y, Zhao Y, Lu C, Fu M, Dou T and Tan X 2015 Signatures of positive selection at hemopexin (PEX) domain of matrix metalloproteinase-9 (MMP-9) gene. J. Biosci.] DOI 10.1007/s12038-015-9577-6

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Liu, Y., Zhao, Y., Lu, C. et al. Signatures of positive selection at hemopexin (PEX) domain of matrix metalloproteinase-9 (MMP-9) gene. J Biosci 40, 885–890 (2015). https://doi.org/10.1007/s12038-015-9577-6

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  • DOI: https://doi.org/10.1007/s12038-015-9577-6

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