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Bifidogenic effect of grain larvae extract on serum lipid, glucose and intestinal microflora in rats

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The main objective of this study was to investigate whether orally administered Korean grain larvae ethanol extract (GLE) had a bifidogenic effect in normal rats. Male Sprague–Dawley rats were divided into a negative control group (CO) and GLE orally administered (5.0, 7.0 and 9.0 mg/100 g body weight) groups. Thymus and spleen weights dose-dependently increased by 128.58% and 128.58%, respectively, but abdominal fat decreased by 19.18% after GLE administration compared with that in the CO group (p<0.05). Serum triglycerides, total cholesterol, low-density lipoprotein cholesterol, and glucose decreased by 30.26%, 7.33%, 27.20%, and 6.96%, respectively, whereas high-density lipoprotein cholesterol increased by 129.93% in the GLE groups compared with those in the CO group (p<0.05). IgG, IgM, IgA in the GLE groups increased 203.68%, 181.41%, and 238.25%, respectively, compared to that in the CO group (p<0.05). Bifidobacteria and Lactobacillus increased by 115.74% and 144.28%, whereas Bacteroides, Clostridium, Escherichia, and Streptococcus decreased by 17.37%, 17.46%, 21.25%, and 19.16%, respectively, in the GLE groups compared with those in the CO group (p<0.05). Total organic acids, acetic acid, and propionic acid increased by151.40%, 188.09%, and 150.17%, whereas butyric acid and valeric acid decreased by 40.65% and 49.24%, respectively, in the GLE groups as compared with those in the CO group (p < 0.05). These results suggest that Korean GLE improves the bifidogenic effect by increasing cecal organic acids and modulating gut microflora via a selective increase in Bifidobacterium in normal rats.

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Acknowledgements

This study was supported by 2012 Kangwon National University (Project No.: C1008543-01-01), and 2014 Post-Doc Program Grant from Kangwon National University, Republic of Korea.

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Authors and Affiliations

  1. Institute of Animal Resources, Kangwon National University, Chuncheon, Gangwondo, 200-701, Korea

    Sang-Oh Park

  2. Division of Applied Animal Science, Kangwon National University, Chuncheon, Gangwondo, 200-701, Korea

    Byung-Sung Park

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  1. Sang-Oh Park
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Correspondence to Byung-Sung Park.

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Corresponding editor: Yogesh Shouche

[Park S-O and Park B-S 2015 Bifidogenic effect of grain larvae extract on serum lipid, glucose and intestinal microflora in rats. J. Biosci.] DOI 10.1007/s12038-015-9540-6

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Park, SO., Park, BS. Bifidogenic effect of grain larvae extract on serum lipid, glucose and intestinal microflora in rats. J Biosci 40, 513–520 (2015). https://doi.org/10.1007/s12038-015-9540-6

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