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BDP-30, a systemic resistance inducer from Boerhaavia diffusa L., suppresses TMV infection, and displays homology with ribosome-inactivating proteins

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Abstract

Root extract of Boerhaavia diffusa L. induced systemic resistance in tobacco against Tobacco mosaic virus. A 30 kDa protein was isolated as the active component, called BDP-30 on the basis of the molecular weight and source plant. BDP-30, a glycoprotein, was found to be temperature and protease resistant. It was basic, possessing a pI greater than 9.0. In-gel proteolytic digestion of BDP-30 generated two peptides that possessed the amino acid sequence KLYDIPPLR and KVTLPYSGNYER by LC/MS/MS. Both peptides shared absolute sequence identity with trichosanthin, a ribosome-inactivating protein from Trichosanthes kirilowii, and a 78% and 100% homology respectively with an RIP from Bryonia dioica, bryodin. Further, effort was made to look at the fate of TMV in induced resistant Nicotiana tabacum cv. Xanthi, a systemic host of the virus, at specified days after inoculation in control and treated plants. TMV coat protein (CP) was detected by immunoblot 7 days post inoculation up to 21 days in the control set, but not in treated resistant plants. TMV RNA was detected by RT-PCR using TMV-CP specific primers. Resistant tobacco did not show presence of TMV RNA up to 21 days of inoculation. This suggests that BDP-30 may be suppressing TMV replication.

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Acknowledgements

SS acknowledges the award of the UGC/CSIR NET JRF no. 276/JRF(UGC)CSIR-1989.

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Author notes

  1. HN Verma

    Present address: Jaipur National University, Jagatpura, Jaipur, 302 017, India

Authors and Affiliations

  1. Molecular Plant Virology Lab, Department of Botany, Lucknow University, Lucknow, 226 007, India

    Shalini Srivastava, HN Verma, Aparana Srivastava & Vivek Prasad

Authors
  1. Shalini Srivastava
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  2. HN Verma
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  3. Aparana Srivastava
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  4. Vivek Prasad
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Correspondence to Vivek Prasad.

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Corresponding editor: Indranil Dasgupta

[Srivastava S, Verma HN, Srivastava A and Prasad V 2015 BDP-30, a systemic resistance inducer from Boerhaavia diffusa L., suppresses TMV infection, and displays homology with ribosome-inactivating proteins. J. Biosci. 40 1–12] DOI 10.1007/s12038-014-9494-0

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Srivastava, S., Verma, H., Srivastava, A. et al. BDP-30, a systemic resistance inducer from Boerhaavia diffusa L., suppresses TMV infection, and displays homology with ribosome-inactivating proteins. J Biosci 40, 125–135 (2015). https://doi.org/10.1007/s12038-014-9494-0

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  • DOI: https://doi.org/10.1007/s12038-014-9494-0

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