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Platelet-derived growth factor mediates interleukin-13-induced collagen I production in mouse airway fibroblasts

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Abstract

Interleukin-13 (IL-13) is associated with the production of collagen in airway remodelling of asthma. Yet, the molecular mechanisms underlying IL-13 induction of collagen remain unclear; the aim of this study is to address this issue. IL-13 dose- and time-dependently-induced collagen I production in primary cultured airway fibroblasts; this was accompanied with the STAT6 phosphorylation, and pre-treatment of cells with JAK inhibitor suppressed IL-13-induced collagen I production. Further study indicated that IL-13 stimulated JAK/STAT6-dependent PDGF production and subsequent ERK1/2 MAPK activation in airway fibroblasts, and the presence of either PDGF receptor blocker or MEK inhibitor partially suppressed IL-13-induced collagen I production. Taken together, our study suggests that activation of JAK/STAT6 signal pathway and subsequent PDGF generation and resultant ERK1/2 MAPK activation mediated IL-13-induced collagen I production in airway fibroblasts.

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Acknowledgements

This work was supported by the Natural Science Foundation of China (81070045), the Key Clinical Project for Affiliated Hospital of Ministry of Public Health of China (111).

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Authors and Affiliations

  1. Respiratory Diseases Research Center, and Department of Respiratory Medicine, The Second Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an, Shaanxi, 710004, P.R. China

    Jiamei Lu, Yanting Zhu, Wei Feng, Yilin Pan, Shaojun Li, Dong Han, Lu Liu, Xinming Xie, Guizuo Wang & Manxiang Li

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  1. Jiamei Lu
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  2. Yanting Zhu
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  3. Wei Feng
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  4. Yilin Pan
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  5. Shaojun Li
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  6. Dong Han
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  7. Lu Liu
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  8. Xinming Xie
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  9. Guizuo Wang
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  10. Manxiang Li
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Corresponding author

Correspondence to Manxiang Li.

Additional information

Corresponding editor: Seyed E Hasnain

[Lu J, Zhu Y, Feng W, Pan Y, Li S, Han D, Liu L, Xie X, Wang G And Li M 2014 Platelet-derived growth factor mediates interleukin-13-induced collagen I production in mouse airway fibroblasts. J. Biosci. 39 1–8] DOI 10.1007/s12038-014-9454-8

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Lu, J., Zhu, Y., Feng, W. et al. Platelet-derived growth factor mediates interleukin-13-induced collagen I production in mouse airway fibroblasts. J Biosci 39, 693–700 (2014). https://doi.org/10.1007/s12038-014-9454-8

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  • DOI: https://doi.org/10.1007/s12038-014-9454-8

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