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Amplicon restriction patterns associated with nitrogenase activity of root nodules for selection of superior Myrica seedlings

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Abstract

Trees of Myrica sp. grow abundantly in the forests of Meghalaya, India. These trees are actinorhizal and harbour nitrogen-fixing Frankia in their root nodules and contribute positively towards the enhancement of nitrogen status of forest areas. They can be used in rejuvenation of mine spoils and nitrogen-depleted fallow lands generated due to slash and burn agriculture practiced in the area. We have studied the association of amplicon restriction patterns (ARPs) of Myrica ribosomal RNA gene and internal transcribed spacer (ITS) region and nitrogenase activity of its root nodules. We found that ARPs thus obtained could be used as markers for early screening of seedlings that could support strains of Frankia that fix atmospheric nitrogen more efficiently.

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Acknowledgements

MY is grateful to Council for Scientific and Industrial Research, India, for providing research fellowship.

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Authors and Affiliations

  1. Molecular Genetics Laboratory, Department of Botany, North-Eastern Hill University, Shillong, 793 022, India

    Mhathung Yanthan & Arvind K Misra

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  1. Mhathung Yanthan
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  2. Arvind K Misra
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Correspondence to Arvind K Misra.

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[Yanthan M and Misra AK 2013 Amplicon restriction patterns associated with nitrogenase activity of root nodules for selection of superior Myrica seedlings. J. Biosci. 38 1–7] DOI 10.1007/s12038-013-9358-z

Supplementary materials pertaining to this article are available on the Journal of Biosciences Website at http://www.ias.ac.in/jbiosci/nov2013/supp/Yanthan.pdf

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Yanthan, M., Misra, A.K. Amplicon restriction patterns associated with nitrogenase activity of root nodules for selection of superior Myrica seedlings. J Biosci 38, 789–795 (2013). https://doi.org/10.1007/s12038-013-9358-z

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  • DOI: https://doi.org/10.1007/s12038-013-9358-z

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