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Sialyl Lewis x expression in cervical scrapes of premalignant lesions

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Abstract

Sialylated oligosaccharides of glycoproteins and glycolipids have been implicated in tumour progression and metastases. Altered expression of glycosidic antigens has been reported in cervical cancer. In cervix premalignant lesions, an increased expression of sialic acid has been reported. In the present study we determined the expression profiles of the glycosidic antigens Tn, sialyl Tn (sTn), Lewis a (Lea), sialyl Lewis a (sLea), Lewis x (Lex) and sialyl Lewis x (sLex) in cervical scrapes with cytological diagnoses of normal, low-grade squamous intraepithelial lesions (LGSIL) and high-grade squamous intraepithelial lesions (HGSIL). Cervical scrapings were collected to detect tumour antigens expressions by flow cytometry using monoclonal antibodies. Cytometry analysis of Tn, sTn, Lea and Lex did not reveal differences at the expression level among groups. The number of positive cells to sLea antigen increased in the HGSIL group with respect to the normal group (p = 0.0495). The number of positive cells to sLex antigen in the samples increased with respect to the grade of squamous intraepithelial lesion (SIL) (p < 0.001, Mann–Whitney U test). The intensity of expression of this antigen increased in the HGSIL samples with respect to normal samples (p < 0.0068).

sLex antigen could be a candidate to be used as biomarker for the early diagnosis of cervical cancer.

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Acknowledgements

This work was supported by grants from the Mexican Institute of Social Security (IMSS FIS/IMSS/PROT/G10/843) and from CONACyT (Salud-C01-2005-13918). NVM is the recipient of a scholarship from the project CONACyT (Salud-C01-2005-13918) and from the Mexican Institute of Social Security.

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Authors and Affiliations

  1. Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km 4.5 Carretera Federal Atlixco-Metepec, Atlixco, Puebla, C.P. 74360, Mexico

    Noé Velázquez-Márquez, Gerardo Santos-López, Julio Reyes-Leyva & Verónica Vallejo-Ruiz

  2. Clínica de Displasias, Hospital General de Zona No. 1, Instituto Mexicano del Seguro Social, Tlaxcala, Tlaxcala, Mexico

    Lucio Jiménez-Aranda

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  1. Noé Velázquez-Márquez
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  2. Gerardo Santos-López
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  3. Lucio Jiménez-Aranda
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  4. Julio Reyes-Leyva
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  5. Verónica Vallejo-Ruiz
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Correspondence to Verónica Vallejo-Ruiz.

Additional information

MS received 14 March 2012; accepted 07 August 2012

Corresponding editor: Rita Mulherkar

[Velázquez-Márquez N, Santos-López G, Jiménez-Aranda L, Reyes-Leyva J and Vallejo-Ruiz V 2012 Sialyl Lewis x expression in cervical scrapes of premalignant lesions. J. Biosci. 37 1–6] DOI 10.1007/s12038-012-9261-z

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Velázquez-Márquez, N., Santos-López, G., Jiménez-Aranda, L. et al. Sialyl Lewis x expression in cervical scrapes of premalignant lesions. J Biosci 37 (Suppl 1), 999–1004 (2012). https://doi.org/10.1007/s12038-012-9261-z

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