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Neuropeptide Y gene expression around meal time in the Brazilian flounder Paralichthys orbignyanus

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Abstract

Neuropeptide Y (NPY) is considered the major stimulant for food intake in mammals and fish. Previous results indicate that NPY is involved in the feeding behaviour of the Brazilian flounder, Paralichthys orbignyanus. In this study, we evaluated hypothalamic NPY expression before (−2 h), during (0 h) and after feeding (+2 h) in two independent experiments: (1) during a normal feeding schedule and (2) in fish fasted for 2 weeks. During normal feeding, changes in the levels of NPY mRNA were periprandial, with expression levels being significantly elevated at meal time (P < 0.05) and significantly reduced 2 h later (P < 0.05). Comparing the fasting and unfasted groups, NPY mRNA levels were significantly higher (P < 0.05) at −2 h and +2 h in the fasting group, but there was no difference at 0 h. In addition, the higher NPY mRNA levels that were observed in the fasting group were maintained throughout the sampling period. In summary, our results show that NPY expression was associated with meal time (0 h) in food intake regulation.

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References

  • Aldegunde M and Mancebo M 2006 Effects of neuropeptide Y on food intake and brain biogenic amines in the rainbow trout (Oncorhynchus mykiss). Peptides 27 719–727

    Article  PubMed  CAS  Google Scholar 

  • Amano M, Okubo K, Yamanome T, Yamada H, Aida K and Yamamori K 2004 Changes in brain GnRH mRNA and pituitary GnRH peptide during testicular maturation in barfin flounder. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 138 435–443

    Article  PubMed  Google Scholar 

  • Campos VF, Collares T, Deschamps JC, Seixas FK, Dellagostin OA, Lanes CF, Sandrini J, Marins LF, et al. 2010 Identification, tissue distribution and evaluation of brain neuropeptide Y gene expression in the Brazilian flounder Paralichthys orbignyanus. J. Biosci. 35 405–413

    Article  PubMed  CAS  Google Scholar 

  • Campos VF, Collares T, Deschamps JC, Seixas FK, Okamoto MH, Sampaio LA, Marins LF and Robaldo RB 2011a Cloning and evaluation of sbGnRH gene expression in juvenile and adult males of Brazilian flounder Paralichthys orbignyanus. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 63 239–246

    Article  CAS  Google Scholar 

  • Campos VF, Collares TV, Seixas FK, Deschamps JC, Marins LFF, Okamoto MH, Sampaio LAN and Robaldo RB 2011b NPY and sbGnRH gene expression in juvenile and adult male Brazilian flounder Paralichthys orbignyanus. Ciencia Rural 41 1927–1930

    Article  CAS  Google Scholar 

  • Carpio Y, Leon K, Acosta J, Morales R and Estrada MP 2007 Recombinant tilapia Neuropeptide Y promotes growth and antioxidant defenses in African catfish (Clarias gariepinus) fry. Aquaculture 272 649–655

    Article  CAS  Google Scholar 

  • Ferretti S, Fornari A, Pedrazzi P, Pellegrini M and Zoli M 2011 Developmental overfeeding alters hypothalamic neuropeptide mRNA levels and response to a high-fat diet in adult mice. Peptides 32 1371–1383

    Article  PubMed  CAS  Google Scholar 

  • Kalra SP, Dube MG, Sahu A, Phelps CP and Kalra PS 1991 Neuropeptide Y secretion increases in the paraventricular nucleus in association with increased appetite for food. Proc. Natl. Acad. Sci. USA 88 10931–10935

    Article  PubMed  CAS  Google Scholar 

  • Kamijo M, Kojima K, Maruyama K, Konno N, Motohashi E, Ikegami T, Uchiyama M, Shioda S, Ando H and Matsuda K 2011 Neuropeptide Y in tiger puffer (Takifugu rubripes): distribution, cloning, characterization, and mRNA expression responses to prandial condition. Zoolog. Sci. 28 882–890

    Article  PubMed  CAS  Google Scholar 

  • Kehoe AS and Volkoff H 2007 Cloning and characterization of neuropeptide Y (NPY) and cocaine and amphetamine regulated transcript (CART) in Atlantic cod (Gadus morhua). Comp. Biochem. Physiol. A Mol. Integr. Physiol. 146 451–461

    Article  PubMed  Google Scholar 

  • Kehoe AS and Volkoff H 2008 The Effects of Temperature on Feeding and Expression of Two Appetite-related Factors, Neuropeptide Y and Cocaine- and Amphetamine-regulated Transcript, in Atlantic Cod, Gadus morhua. J. World Aquaculture Soc. 39 790–796

    Article  Google Scholar 

  • Lanes CFC, Okamoto M, Cavalcanti PV, Collares T, Campos VF, Deschamps JC, Robaldo RB, Marins LF and Sampaio LA 2008 Cryopreservation of Brazilian flounder (Paralichthys orbignyanus) sperm. Aquaculture 275 361–365

    Article  CAS  Google Scholar 

  • Lanes CFC, Okamoto MH, Bianchini A, Marins LF and Sampaio LA 2010 Sperm quality of Brazilian flounder Paralichthys orbignyanus throughout the reproductive season. Aquaculture Res. 41 e199–e207

    Article  CAS  Google Scholar 

  • Lanes CFC, Sampaio LA and Marins LF 2009 Evaluation of DNase activity in seminal plasma and uptake of exogenous DNA by spermatozoa of the Brazilian flounder Paralichthys orbignyanus. Theriogenology 71 525–533

    Article  PubMed  CAS  Google Scholar 

  • Livak KJ and Schmittgen TD 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods 25 402–408

    Article  PubMed  CAS  Google Scholar 

  • Lopez-Patino MA, Guijarro AI, Isorna E, Delgado MJ, Alonso-Bedate M and de Pedro N 1999 Neuropeptide Y has a stimulatory action on feeding behavior in goldfish (Carassius auratus). Eur. J. Pharmacol. 377 147–153

    Article  PubMed  CAS  Google Scholar 

  • MacDonald E and Volkoff H 2009 Neuropeptide Y (NPY), cocaine- and amphetamine-regulated transcript (CART) and cholecystokinin (CCK) in winter skate (Raja ocellata): cDNA cloning, tissue distribution and mRNA expression responses to fasting. Gen. Comp. Endocrinol. 161 252–261

    Article  PubMed  CAS  Google Scholar 

  • Meier KM, Figueiredo MA, Kamimura MT, Laurino J, Maggioni R, Pinto LS, Dellagostin OA, et al. 2009 Increased growth hormone (GH), growth hormone receptor (GHR) and insulin-like growth factor I (IGF-I) gene transcription after hyperosmotic stress in the Brazilian flounder Paralichthys orbignyanus. Fish Physiol. Biochem. 35 501–509

    Article  PubMed  CAS  Google Scholar 

  • Narnaware YK and Peter RE 2001 Effects of food deprivation and refeeding on neuropeptide Y (NPY) mRNA levels in goldfish. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 129 633–637

    Article  CAS  Google Scholar 

  • Narnaware YK, Peyon PP, Lin XW and Peter RE 2000 Regulation of food intake by neuropeptide Y in goldfish. Am. J. Physiol. Reg. Integr. Comp. Physiol. 279 R1025–R1034

    CAS  Google Scholar 

  • Radonic M, Muller MI, Lopez AV, Bambill GA, Spinedi M and Boccanfuso JJ 2007 Improvement in flounder Paralichthys orbignyanus controlled spawning in Argentina. Ciencias Marinas 33 187–196

    Google Scholar 

  • Sahu A, White JD, Kalra PS and Kalra SP 1992 Hypothalamic neuropeptide Y gene expression in rats on scheduled feeding regimen. Brain Res. Mol. Brain Res. 15 15–18

    Article  PubMed  CAS  Google Scholar 

  • Sampaio LA, Freitas LS, Okamoto MH, Louzada LR, Rodrigues RV and Robaldo RB 2007 Effects of salinity on Brazilian flounder Paralichthys orbignyanus from fertilization to juvenile settlement. Aquaculture 262 340–346

    Article  CAS  Google Scholar 

  • Sampaio LA, Robaldo RB and Bianchini A 2008 Hormone-induced ovulation, natural spawning and larviculture of Brazilian flounder Paralichthys orbignyanus (Valenciennes, 1839). Aquaculture Res. 39 712–717

    Article  Google Scholar 

  • Saneyasu T, Honda K, Kamisoyama H, Ikura A, Nakayama Y and Hasegawa S 2011 Neuropeptide Y effect on food intake in broiler and layer chicks. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 159 422–426

    Article  PubMed  Google Scholar 

  • Silverstein JT and Plisetskaya EM 2000 The effects of NPY and insulin on food intake regulation in fish. Am. Zool. 40 296–308

    Article  CAS  Google Scholar 

  • Sucajtys-Szulc E, Goyke E, Korczynska J, Stelmanska E, Rutkowski B and Swierczynski J 2008 Chronic food restriction differentially affects NPY mRNA level in neurons of the hypothalamus and in neurons that innervate liver. Neurosci. Lett. 433 174–177

    Article  PubMed  CAS  Google Scholar 

  • Valassi E, Scacchi M and Cavagnini F 2008 Neuroendocrine control of food intake. Nutr. Metab. Cardiovasc. Dis. 18 158–168

    Article  PubMed  CAS  Google Scholar 

  • Volkoff H, Hoskins LJ and Tuziak SM 2010 Influence of intrinsic signals and environmental cues on the endocrine control of feeding in fish: potential application in aquaculture. Gen. Comp. Endocrinol. 167 352–359

    Article  PubMed  CAS  Google Scholar 

  • Volkoff H, Xu MY, MacDonald E and Hoskins L 2009 Aspects of the hormonal regulation of appetite in fish with emphasis on goldfish, Atlantic cod and winter flounder: Notes on actions and responses to nutritional, environmental and reproductive changes. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 153 8–12

    Article  PubMed  Google Scholar 

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Acknowledgements

This work was supported by MCT/CNPq-Edital Universal (# 47438/2006-7). RBR, JCD, AJAMcB, LFM and LAS are research fellows of CNPq.

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Authors and Affiliations

  1. Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, CP 354, 96010-900, Pelotas, RS, Brazil

    Vinicius F Campos, João C Deschamps, Fabiana K Seixas, Alan John A McBride & Tiago Collares

  2. Departamento de Fisiologia e Farmacologia, Instituto de Biologia, Universidade Federal de Pelotas, CP 354, 96010-900, Pelotas, RS, Brazil

    Ricardo B Robaldo

  3. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, CP 474, 96201-900, Rio Grande, RS, Brazil

    Luis Fernando Marins

  4. Laboratório de Piscicultura Estuarina e Marinha, Instituto de Oceanografia, Universidade Federal do Rio Grande, CP 474, 96201-900, Rio Grande, RS, Brazil

    Marcelo Okamoto & Luís A Sampaio

Authors
  1. Vinicius F Campos
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  2. Ricardo B Robaldo
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  3. João C Deschamps
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  4. Fabiana K Seixas
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  5. Alan John A McBride
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  6. Luis Fernando Marins
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  7. Marcelo Okamoto
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  8. Luís A Sampaio
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  9. Tiago Collares
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Corresponding author

Correspondence to Vinicius F Campos.

Additional information

Corresponding editor: Raphael Pinaud

MS received 15 November 2011; accepted 19 April 2012

Corresponding editor: Raphael Pinaud

[Campos VF, Robaldo RB, Deschamps JC, Seixas FK, McBride AJA, Marins LF, Okamoto M, Sampaio LA and Collares T 2012 Neuropeptide Y gene expression around meal time in the Brazilian flounder Paralichthys orbignyanus. J. Biosci. 37 1–6] DOI 10.1007/s12038-012-9205-7

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Campos, V.F., Robaldo, R.B., Deschamps, J.C. et al. Neuropeptide Y gene expression around meal time in the Brazilian flounder Paralichthys orbignyanus . J Biosci 37, 227–232 (2012). https://doi.org/10.1007/s12038-012-9205-7

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  • DOI: https://doi.org/10.1007/s12038-012-9205-7

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