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Mutagenesis in ORF AV2 affects viral replication in Mungbean yellow mosaic India virus

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Abstract

Mungbean yellow mosaic India virus (MYMIV) is a whitefly-transmitted begomovirus with a bipartite genome. We investigate the functions of the MYMIV-AV2 protein, the open reading frame present upstream of the coat protein gene in DNA A component. The ability of MYMIV-AV2 mutants to replicate, spread and cause symptoms in legume hosts, blackgram, cowpea and French bean was analysed. Plants agroinoculated with mutants K73R, C86S and the double mutant C84S,C86S showed increase in severity of symptoms compared with the wild type. However, mutants W2S and H14Q,G15E caused marked attenuation of symptoms. While the double mutants C84S,C86S caused a 50-fold increase in double-stranded supercoiled and single-stranded DNA accumulation, the mutations W2S and H14Q,G15E showed a decrease in double-stranded supercoiled and single-stranded viral DNA accumulation. Because AV2 mutants affect the ratio between open circular and supercoiled DNA forms, we hypothesize that these mutations may modulate the functions of the replication initiation protein.

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Abbreviations

ACMV:

Arican cassava mosaic virus

ds:

double-stranded

EACMCV:

East African cassava mosaic Cameroon virus

MYMIV:

Mungbean yellow mosaic India virus

MYMV:

Mungbean yellow mosaic virus

MYMV-Vig:

Mungbean yellow mosaic virus – Vigna

ORF:

open reading frame

PKC:

protein kinase C

PTGS:

post-transcriptional gene silencing

PTR:

partial head to tail repeat

PVX:

Potato virus X

Ss:

single-stranded

ToLCNDV:

Tomato leaf curl New Delhi virus

TYLCV-Is:

Tomato yellow leaf curl virus – Israel

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Acknowledgements

The financial support given by Department of Biotechnology, India, is gratefully acknowledged. We are thankful to the director, Indian Agricultural Research Institute, and the head, Division of Plant Pathology, Indian Agricultural Research Institute, India, for providing necessary facilities. We are also grateful to the scientists and staff at National Phytotron Facility, Indian Agricultural Research Institute, India, for their guidance and support in growing plants under controlled conditions.

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Authors and Affiliations

  1. Department of Horticulture, Agriculture Faculty, Ilam University, Ilam, Iran

    A Rouhibakhsh

  2. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110 067, India

    Q M I Haq

  3. Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    V G Malathi

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  1. A Rouhibakhsh
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  2. Q M I Haq
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  3. V G Malathi
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Correspondence to V G Malathi.

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[Rouhibakhsh A Haq QMI and Malathi VG 2011 Mutagenesis in ORF AV2 affects viral replication in Mungbean yellow mosaic India virus. J. Biosci. 36 329–340] DOI

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Rouhibakhsh, A., Haq, Q.M.I. & Malathi, V.G. Mutagenesis in ORF AV2 affects viral replication in Mungbean yellow mosaic India virus . J Biosci 36, 329–340 (2011). https://doi.org/10.1007/s12038-011-9041-1

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