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Journal of Biosciences

, Volume 36, Issue 1, pp 37–42 | Cite as

Heat-inducible Cre-lox system for marker excision in transgenic rice

  • Abhilasha Khattri
  • Soumen Nandy
  • Vibha SrivastavaEmail author
Brief communication

Abstract

The present study assessed the efficacy of a heat-inducible cre gene for conditional removal of the marker gene from a rice genome via Cre-lox recombination. A cre gene controlled by the soybean heat-shock promoter was introduced into the rice genome along with the recombination target (lox) construct. Cre-mediated recombination was expected to remove the marker gene and activate the promoter-less GUS gene. Six transgenic lines displayed well-regulated heat-inducible Cre activity in the callus. However, only one line that contained a single copy of the cre gene maintained this property in the regenerated plants and their progeny. Marker-free progeny were obtained from the plant that was heat-treated at the seedling stage, indicating the inheritance of the recombination ‘footprint’. The presence of the ‘footprint’ was verified by polymerase chain reaction and Southern analysis. Therefore, the cre gene controlled by the soybean heat-shock promoter is an effective tool for conditional removal of the marker gene in rice.

Keywords

Cre-lox inducible promoter marker removal site-specific recombination transgenic rice 

Notes

Acknowledgements

This work was supported by the USDA-CSREES grant (BRAG 2006-33120-17718), and the ABI-Arkansas Division of Agriculture grant to VS. We thank Dr Yinong Yang and Dr David Ow for the gift of HSP promoter and cre gene, respectively.

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Copyright information

© Indian Academy of Sciences 2011

Authors and Affiliations

  • Abhilasha Khattri
    • 1
  • Soumen Nandy
    • 1
  • Vibha Srivastava
    • 1
    • 2
    Email author
  1. 1.Department of Crop, Soil and Environmental SciencesUniversity of ArkansasFayettevilleUSA
  2. 2.Department of HorticultureUniversity of ArkansasFayettevilleUSA

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