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Translocations used to generate chromosome segment duplications in Neurospora can disrupt genes and create novel open reading frames

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Abstract

In Neurospora crassa, crosses between normal sequence strains and strains bearing some translocations can yield progeny bearing a duplication (Dp) of the translocated chromosome segment. Here, 30 breakpoint junction sequences of 12 Dp-generating translocations were determined. The breakpoints disrupted 13 genes (including predicted genes), and created 10 novel open reading frames. Insertion of sequences from LG III into LG I as translocation T(UK8-18) disrupts the eat-3 gene, which is the ortholog of the Podospora anserine gene ami1. Since ami1-homozygous Podospora crosses were reported to increase the frequency of repeat-induced point mutation (RIP), we performed crosses homozygous for a deficiency in eat-3 to test for a corresponding increase in RIP frequency. However, our results suggested that, unlike in Podospora, the eat-3 gene might be essential for ascus development in Neurospora. Duplication-heterozygous crosses are generally barren in Neurospora; however, by using molecular probes developed in this study, we could identify Dp segregants from two different translocation-heterozygous crosses, and using these we found that the barren phenotype of at least some duplication-heterozygous crosses was incompletely penetrant.

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Abbreviations

Dp :

duplication

Df:

deficiency

ITs:

insertional translocations

LG:

lineage group

OR:

Oak Ridge

ORFs:

open reading frames

PCR:

polymerase chain reaction

QTs:

quasiterminal translocations

RIP:

repeat-induced point mutation

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Authors and Affiliations

  1. Centre for Cellular and Molecular Biology, Hyderabad, 500 007, India

    Parmit K. Singh, Srividhya V. Iyer, T. Naga Sowjanya, B. Kranthi Raj & Durgadas P. Kasbekar

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  1. Parmit K. Singh
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  2. Srividhya V. Iyer
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  3. T. Naga Sowjanya
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  4. B. Kranthi Raj
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  5. Durgadas P. Kasbekar
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Correspondence to Durgadas P. Kasbekar.

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Dedicated to the memory of our colleague T Bhavani Prasanna.

Supplementary figure pertaining to this article is available on the Journal of Biosciences Website at http://www.ias.ac.in/jbiosci/Dec2010/pp539–546/suppl.pdf

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Singh, P.K., Iyer, S.V., Naga Sowjanya, T. et al. Translocations used to generate chromosome segment duplications in Neurospora can disrupt genes and create novel open reading frames. J Biosci 35, 539–546 (2010). https://doi.org/10.1007/s12038-010-0062-y

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  • DOI: https://doi.org/10.1007/s12038-010-0062-y

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