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Antimicrobial, antioxidant, cytotoxicity and platelet aggregation inhibitory activity of a novel molecule isolated and characterized from mango ginger (Curcuma amada Roxb.) rhizome

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Abstract

Mango ginger (Curcuma amada Roxb.) rhizome is used in the manufacture of pickles and other food preparations due to its unique raw mango flavour. The chloroform extract of mango ginger rhizome was subjected to antibacterial activity-guided purification by repeated silica gel column chromatography to obtain a pure compound. The structure of the isolated compound was deduced by analysing UV, IR, LC-MS and 2D-HMQCT NMR spectral data, and named it as amadaldehyde, a novel compound. It exhibited a wide range of antibacterial activity with potential bactericidal activity against several bacteria. The purified compound also exhibited antioxidant activity, cytotoxicity and platelet aggregation inhibitory activities.

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Abbreviations

ADP:

adenosine diphosphate

BHA:

butylated hydroxy anisole

DMEM:

Dulbecco modified Eagle medium

DMEMPR:

Dulbecco modified Eagle medium without phenol red

DMRT:

Duncan multiple range test

DMSO:

dimethyl sulphoxide

DPPH:

1,1-diphenyl-2-picryl-hydrazyl

EDTA:

ethylene diamine tetra-acetic acid

ESI:

electrospray ionization

Fr.:

fraction

HPLC:

high performance liquid chromatography

IR:

infra red

LC-MS:

liquid chromatography-mass spectrometry

MBC:

minimum bactericidal concentration

MIC:

minimum inhibitory concentration

MTT:

3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide

NADH:

nicotinamide adenine dinucleotide

NBCS:

newborn calf serum

NBT:

nitroblue tetrazolium

NMR:

nuclear magnetic resonance

OD:

optical density

PMS:

phenazine methosulphate

PPP:

platelet-poor plasma

PRP:

platelet-rich plasma

SRB:

sulphorhodamine B

TBA:

thiobarbituric acid

TCA:

trichloroacetic acid

TLC:

thin-layer chromatography

UV:

ultraviolet

References

  • Alzoreky N S and Nakahara K 2003 Antibacterial activity of extracts from some edible plants commonly consumed in Asia; Int. J. Food Microbiol. 80 223–230

    Article  CAS  PubMed  Google Scholar 

  • Blois M S 1958 Antioxidant determinations by use of a stable free radical; Nature (London) 181 1199–1200

    Article  CAS  Google Scholar 

  • Chen C W and Ho C T 1995 Antioxidant properties of polyphenols extracted from green and black tea; J. Food Lipids 2 35–46

    Article  CAS  Google Scholar 

  • Council of Scientific and Industrial Research (CSIR) 1950 Wealth of India — raw materials. vol. 2 (New Delhi: CSIR) p. 401

    Google Scholar 

  • Cowan M M 1999 Plant products as antimicrobial agents; Clin. Microbiol. Rev. 12 564

    CAS  PubMed  Google Scholar 

  • Dahl M K and Richardson T 1978 Photogeneration of superoxide anion in serum of bovine milk and in model systems containing riboflavin and amino acids; J. Dairy Sci. 61 400–407

    Article  Google Scholar 

  • Decker E A and Welch B 1990 Role of ferritin as a lipid oxidation catalyst in muscle food; J. Agric. Food Chem. 38 674–677

    Article  CAS  Google Scholar 

  • Dinerman J L and Mehta J L 1990 Endothelial, platelet and leukocyte interactions in ischemic heart disease: insights into potential mechanisms and their clinical relevance; J. Am. Cardiol. 16 207–222

    Article  CAS  Google Scholar 

  • Dinis T C P, Almeida L M and Madeira V M C 1994 Action of phenolic derivatives (acetaminophen, salicylate and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers; Arch. Biochem. Biophys. 315 161–169

    Article  CAS  PubMed  Google Scholar 

  • Duh P D, Tu Y Y and Yen G C 1999 Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat); LWT Food Sci. Technol. 32 269–277

    CAS  Google Scholar 

  • Duh P D and Yen G H 1997 Antioxidative activity of three-herbal water extracts; Food Chem. 60 639–645

    Article  CAS  Google Scholar 

  • Francis D and Rita L 1986 Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability; J. Immunol. Methods 89 271–277

    Article  Google Scholar 

  • Fridovich I 1989 Superoxide dismutases. An adaptation to a paramagnetic gas; J. Biol. Chem. 264 7761–7764

    CAS  PubMed  Google Scholar 

  • Halliwell B and Gutteridge J M C 1990 Role of free radicals and catalytic metal ions in human disease: an overview; Methods Enzymol. 186 1–85

    Article  CAS  PubMed  Google Scholar 

  • Heim K E, Tagliaferro A R and Bobilya D J 2002 Flavonoid antioxidants: chemistry and structure-activity relationships; J. Nutr. Biochem. 13 572–584

    Article  CAS  PubMed  Google Scholar 

  • Hirsh J 1987 Hyperactive platelets and complications of coronary artery disease; New Engl. J. Med. 316 1543–1544

    Article  CAS  PubMed  Google Scholar 

  • Hussain A, Virmani O P, Popli S P, Misra L N and Gupta M M 1992 Dictionary of Indian medicinal plants (Lucknow: CIMAP) p. 161

    Google Scholar 

  • Jones R N, Barry A L, Gavan T L and Washington J A I 1985 Microdilution and macrodilution broth procedures; in Manual of clinical microbiology (eds.) E H Lennette, A Balows, W J Hausler Jr and H J Shadomy (Washington, DC: American Society for Microbiology) p. 972.

    Google Scholar 

  • Kirtikar K R and Basu B D 1984 Indian medicinal plants, vol. 4, second edition (Dehra Dun: Bishen Singh Mahendra Pal Singh) pp 2422–2423

    Google Scholar 

  • Krieg N R and Holt J G 1984 Bergey’s manual of systematic bacteriology, vol. 1, 4th edition (Baltimore: Williams and Wilkins)

    Google Scholar 

  • Meyer A S and Isaksen A 1995 Application of enzymes as food antioxidants; Trends Food Sci. Technol. 6 300–304

    Article  CAS  Google Scholar 

  • Moon J H and Terao J 1998 Antioxidant activity of caffeic acid and dihydrocaffeic acid in lard and human low-density lipoprotein; J. Agric. Food Chem. 46 5062–5065

    Article  CAS  Google Scholar 

  • Mukherjee P K, Balasubramanian R, Saha K, Saha B P and Pal M 1995 Antibacterial efficiency of Nelumbo nucifera (Nymphaeaceae) rhizomes extract; Indian Drugs 32 274–276

    Google Scholar 

  • Naigre R, Kalck P, Rouques C, Roux I and Michel G 1996 Comparision of antimicrobial properties of monoterpenes and their carbonylated products; Planta Medica 62 275

    Article  CAS  PubMed  Google Scholar 

  • Nasar-Abbas SM and Kadir Halkman A 2004 Antimicrobial effect of water extract of sumac (Rhus coriaria L.) on the growth of some food borne bacteria including pathogens; Int. J. Food Microbiol. 97 63–69

    Article  CAS  PubMed  Google Scholar 

  • Nikolaos N, Hong-yu Z and Maria Z 2003 Structure-antioxidant activity relationship of ferulic acid derivatives: effect of carbon side chain characteristic groups; J. Agric. Food Chem. 51 1874–1879

    Article  Google Scholar 

  • Nishikimi M, Rao N A and Yagi K 1972 The occurrence of super oxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen; Biochem. Biophys. Res. Commun. 46 849–864

    Article  CAS  PubMed  Google Scholar 

  • Peter H A S, Nicolas S, Mair M, E Sharpe and John G H 1986 Bergey’s manual of systematic bacteriology, vol. 2 (Baltimore: Williams and Wilkins)

    Google Scholar 

  • Philip S, Rista S, Dominic S, Anne M, James M, David V, Jonathan TW, Heidi B et al. 1990 New colorimetric cytotoxic assay for anticancer drug screening; J. Natl. Cancer Inst. 82 1107–1112

    Article  Google Scholar 

  • Policegoudra R S, Abiraj K, Channe Gowda D and Aradhya S M 2007b Isolation and characterization of antioxidant and antibacterial compound from mango ginger (Curcuma amada Roxb.) rhizome; J. Chromatogr. B 854 40–48

    Article  Google Scholar 

  • Policegoudra R S, Divakar S and Aradhya S M 2007a Identification of difurocumenonol, a novel antimicrobial compound from mango ginger (Curcuma amada Roxb.) rhizome; J. Appl. Microbiol. 102 1596–1602

    Article  Google Scholar 

  • Sikkema J, Bont J A M and Poolman B 1995 Mechanisms of membrane toxicity of hydrocarbons; Microbiol. Rev. 59 201–222

    CAS  PubMed  Google Scholar 

  • Sikkema J, de Bont J A M and Poolman B 1994 Interactions of cyclic hydrocarbons with biological membranes; J. Biol. Chem. 269 8022–8028

    CAS  PubMed  Google Scholar 

  • Sikkema J, Poolman B, Konings W N and de Bont J A M 1992 Effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes; J. Bacteriol. 174 2986–2992

    CAS  PubMed  Google Scholar 

  • Smith-Palmer A, Stewart J and Fyfe L 1998 Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens; Lett. Appl. Microbiol. 26 118–122

    Article  CAS  PubMed  Google Scholar 

  • Warrier P K, Nambiar V P K and Ramankutty C 1994 Indian medicinal plants — a compendium of 500 species, vol. 1 (Chennai: Orient Longman Pvt. Ltd) p. 106

    Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Defence Research Laboratory, Tezpur, 784 001, India

    R. S. Policegoudra

  2. Department of Fruit and Vegetable Technology, Central Food Technological Research Institute, Mysore, 570 020, India

    K. Rehna & S. M. Aradhya

  3. Plantation Products, Spices and Flavour Technology, Central Food Technological Research Institute, Mysore, 570 020, India

    L. Jaganmohan Rao

Authors
  1. R. S. Policegoudra
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  2. K. Rehna
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  3. L. Jaganmohan Rao
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  4. S. M. Aradhya
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Correspondence to S. M. Aradhya.

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Policegoudra, R.S., Rehna, K., Jaganmohan Rao, L. et al. Antimicrobial, antioxidant, cytotoxicity and platelet aggregation inhibitory activity of a novel molecule isolated and characterized from mango ginger (Curcuma amada Roxb.) rhizome. J Biosci 35, 231–240 (2010). https://doi.org/10.1007/s12038-010-0027-1

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