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Inhibition of human lung adenocarcinoma growth using survivint34a by low-dose systematic administration

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Abstract

Anti-apoptosis plays an important role in tumour formation and development. Survivin is a member of the inhibitor of apoptosis (IAP) family, which is a target for anti-cancer drug exploitation was replaced as development. We investigated the role of the homo dominant-negative mutant Survivin-T34A in suppressing human lung adenocarcinomas (A549). The anti-tumour activity of HSurvivinT34A plasmid was evaluated in the A549 cell line and nude mice bearing A549 subcutaneous tumours. Low-dose systemic administration was continuously used. The HSurvivinT34A plasmid (5 µg/one) complexed with a cationic liposome (DOTAP/Chol) significantly inhibited tumour growth in our model. We observed microvessel density degradation by CD31 immunohistochemistry and apoptotic cell increase by TUNEL assay, PI staining and flow cytometric analysis in the treated group. The present findings suggest that the HSurvivinT34A plasmid complexed with a cationic liposome may provide an effective approach to inhibit the growth of human lung adenocarcinomas in vitro and in vivo.

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Abbreviations

ANOVA:

analysis of variance

FBS:

foetal bovine serum

H&E:

haematoxylin and eosin

IAP:

inhibitor of apoptosis

MVD:

microvessel density

NSCLC:

non-small cell lung cancer

PCR:

polymerase chain reaction

PI:

propidium iodine

P-S:

penicillin/streptomycin

TAL:

Tachypleus amebocyte lysate

TUNEL:

terminal eoxynucleotidyltransferase-mediated dUTP nick-end labelling

VEGF:

vascular endothelial growth factor

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Authors and Affiliations

  1. State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Keyuan Road 4, Chengdu, Sichuan, 610041, China

    Yan Shan, Chunting Wang, Li Yang, Li Juan Chen, Hong Xin Deng, Han Shuo Yang, Zhimian Li, Zhiyong Li, Li Pan, Fei Leng & Yuquan Wei

Authors
  1. Yan Shan
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  2. Chunting Wang
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  3. Li Yang
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  4. Li Juan Chen
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  5. Hong Xin Deng
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  6. Han Shuo Yang
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  7. Zhimian Li
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  8. Zhiyong Li
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  9. Li Pan
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  10. Fei Leng
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  11. Yuquan Wei
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Corresponding author

Correspondence to Chunting Wang.

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These authors contributed equally to this research.

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Shan, Y., Wang, C., Yang, L. et al. Inhibition of human lung adenocarcinoma growth using survivint34a by low-dose systematic administration. J Biosci 35, 209–216 (2010). https://doi.org/10.1007/s12038-010-0025-3

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  • DOI: https://doi.org/10.1007/s12038-010-0025-3

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