Abstract
Studies that investigate the radiation of human tumour xenografts require an appropriate radiation source and highly standardized conditions during radiation. This work reports on the design of a standardized irradiation device using a commercially available X-ray tube with a custom constructed lead collimator with two circular apertures and an animal bed plate, permitting synchronous irradiation of two animals. Dosimetry and the corresponding methodology for radiotherapy of human non-small cell lung cancer xenograft tumours transplanted to and growing subcutaneously on the right lower limb in a nude rat model were investigated. Procedures and results described herein prove the feasibility of use of the device, which is applicable for any investigation involving irradiation of non-tumorous and tumorous lesions in small animals.
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Tokalov, S.V., Enghardt, W. & Abolmaali, N. Tumour bed irradiation of human tumour xenografts in a nude rat model using a common X-ray tube. J Biosci 35, 203–207 (2010). https://doi.org/10.1007/s12038-010-0024-4
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DOI: https://doi.org/10.1007/s12038-010-0024-4