Journal of Biosciences

, Volume 33, Issue 2, pp 279–287

Nano-composition of riboflavin-nafion functional film and its application in biosensing

  • S. Rezaei-Zarchi
  • A. A. Saboury
  • A. Javed
  • A. Barzegar
  • S. Ahmadian
  • A. Bayandori-Moghaddam


A novel nafion-riboflavin membrane was constructed and characterized by the scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy and cyclic voltammetric techniques. The estimated average diameter of the designed nanoparticles was about 60 nm. The functional membrane showed a quasi-reversible electrochemical behaviour with a formal potential of −562 ± 5 mV (vs Ag/AgCl) on the gold electrode. Some electrochemical parameters were estimated, indicating that the system has good and stable electron transfer properties. Moreover, horseradish peroxidase (HRP) was immobilized on the riboflavin-nafion functional membrane. The electrochemical behaviour of HRP was quasi-reversible with a formal potential of 80 ± 5 mV (vs Ag/AgCl). The HRP in the film exhibited good catalytic activity towards the reduction of H2O2. It shows a linear dependence of its cathodic peak current on the concentration of H2O2, ranging from 10 to 300 µM.


Biosensor functional membrane horseradish peroxidase nanoparticles 

Abbreviations used


horseradish peroxidase


nafion perfluorosulphonated ion-exchange resin


scanning electron microscopy


transmission electron microscopy


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Copyright information

© Indian Academy of Sciences 2008

Authors and Affiliations

  • S. Rezaei-Zarchi
    • 1
    • 2
  • A. A. Saboury
    • 1
  • A. Javed
    • 3
  • A. Barzegar
    • 1
  • S. Ahmadian
    • 1
  • A. Bayandori-Moghaddam
    • 4
  1. 1.Institute of Biochemistry and BiophysicsUniversity of TehranTehranIran
  2. 2.Department of BiologyPayam-e-Noor UniversityYazdIran
  3. 3.Department of BiochemistryUniversity of AgricultureFaisalabadPakistan
  4. 4.Department of Chemistry, Faculty of SciencesUniversity of TehranTehranIran

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