Abstract
The present study aimed to elucidate the effect of nutritional alteration on biomass content and azadirachtin production in cell suspensions of the elite neem variety crida-8. Variations in total nitrogen availability in the medium in terms of different ratios of nitrate:ammonium showed that the ratio 4:1 revealed a profound effect, leading to a 1.5-fold increase in the total extracellular azadirachtin production (5.59 mg/l) over the standard MS medium. Reduction in sucrose (15 mg/l) in the medium exhibited a reduction in biomass and absence of azadirachtin, whereas total phosphate reduction raised intracellular azadirachtin production (6.98 mg/l). An altered medium with a nitrate:ammonium ratio of 4:1 coupled with complete elimination of phosphate enhanced biomass by 36% (59.36 g/l).
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Abbreviations
- BAP:
-
benzyl aminopurine
- DMRT:
-
Duncan multiple range test
- HPLC:
-
high performance liquid chromatography
- K:
-
kinetin
- NAA:
-
naphthalene acetic acid
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Sujanya, S., Poornasri Devi, B. & Sai, I. In vitro production of azadirachtin from cell suspension cultures of Azadirachta indica . J Biosci 33, 113–120 (2008). https://doi.org/10.1007/s12038-008-0027-6
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DOI: https://doi.org/10.1007/s12038-008-0027-6