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Heterotrophic free-living and particle-bound bacterial cell size in the river Cauvery and its downstream tributaries

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Abstract

This is the first comprehensive study on planktonic heterotrophic bacterial cell size in the river Cauvery and its important tributaries in Karnataka State, India. The initial hypothesis that the mean cell size of planktonic heterotrophic bacteria in the four tributaries are markedly different from each other and also from that in the main river Cauvery was rejected, because all five watercourses showed similar planktonic heterotrophic bacterial cell size. Examination of the correlation between mean heterotrophic bacterial cell size and environmental variables showed four correlations in the river Arkavathy and two in the river Shimsha. Regression analysis revealed that 18% of the variation in mean heterotrophic free-living bacterial cell size was due to biological oxygen demand (BOD) in the river Arkavathy, 11% due to surface water velocity (SWV) in the river Cauvery and 11% due to temperature in the river Kapila. Heterotrophic particle-bound bacterial cell size variation was 28% due to chloride and BOD in the river Arkavathy, 11% due to conductivity in the river Kapila and 8% due to calcium in the river Cauvery. This type of relationship between heterotrophic bacterial cell size and environmental variables suggests that, though the mean heterotrophic bacterial cell size was similar in all the five water courses, different sets of environmental variables apparently control the heterotrophic bacterial cell size in the various water bodies studied in this investigation. The possible cause for this environmental (bottom-up) control is discussed.

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Abbreviations

BOD:

biological oxygen demand

SWV:

surface water velocity

FLB:

free-living bacteria

PBB:

particle-bound bacteria

CFU:

colony-forming unit

AODC:

acridine orange direct count

References

  • APHA 1992 Standard methods for examination of water and waste water (Washington, D C: American Public Health Association) 18th edition, pp 1–1000

    Google Scholar 

  • Bennet S J, Sanders R W and Porter K G 1990 Heterotrophic, autotrophic and mixotrophic nanoflagellates: seasonal abundance and bacterivory in a eutrophic lake; Limnol. Oceanogr. 35 1821–832

    Article  Google Scholar 

  • Bird D F and Kalff J 1984 Empirical relationships between bacterial abundance and chlorophyll concentration in fresh and marine waters; Can. J. Fish. Aquat. Sci. 41 1015–1023

    Article  Google Scholar 

  • Callieri C and Heinimaa S 1997 Microbial loop in the large sub alpine lakes; Mem. Ist. Ital. Idrobiol. 56 143–156

    Google Scholar 

  • Cole J J, Pace L M, Caraco F N and Steinhart S G 1993 Bacterial biomass and cell-size distribution in lakes: more and larger cells in anoxic waters; Limnol. Oceanogr. 38 1627–1632

    Article  Google Scholar 

  • Ekebom J 1999 Heterotrophic nanoflagellates and bacteria in sediment of a brackish water sill basin in the Baltic Sea; Hydrobilogia 393 151–161

    Article  Google Scholar 

  • Felip M, Pace M L and Cole J J 1996 Regulation of planktonic bacteria growth rates: the effects of temperature and resources; Microb. Ecol. 31 15–28

    Article  CAS  Google Scholar 

  • Findlay S, Pace L M, Lints D and Cole J J 1991 Weak coupling of bacterial and algal production in a heterotrophic ecosystem: the Hudson river estuary; Limnol. Oceanogr. 36 268–278

    Article  Google Scholar 

  • Fry J C 1990 Direct methods and biomass estimation; Methods Microbiol. 22 41–85

    Article  Google Scholar 

  • Garnier J, Billen G and Servais P 1992 Physiological characteristics and ecological role of small and large sized bacteria in a polluted river (Seine River, France); Arch. Hydrobiol. Beih. 37 83–94

    Google Scholar 

  • Gasol J M and Vaque D 1993 Lack of coupling between heterotrophic nanoflagellates and bacteria: a general phenomenon across aquatic systems?; Limnol. Oceanogr. 38 657–665

    Article  Google Scholar 

  • González J M, Sherr E B and Sherr B F 1990 Size selective grazing on bacteria by natural assemblages of estuarine flagellates and ciliates; Appl. Environ. Microbiol. 56 583–589

    PubMed  PubMed Central  Google Scholar 

  • Hadas O, Pinkas R, Albert-Diez C, Bloem J, Cappenberg T and Berman T 1990 The effect of detrital addition on the development of nanoflagellates and bacteria in lake Kinneret; J. Plankton Res. 12 185–199

    Article  Google Scholar 

  • Hahn M W and Höfle M G 1999 Flagellate predation on a bacterial model community: interplay of size-selective grazing, specific bacterial cell size, and bacterial community composition; Appl. Environ. Microbiol. 65 4863–4872

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hahn M W, Moore E R B and Höfle M G 1999 Bacterial filament formation, a defence mechanism against flagellate grazing, is growth rate controlled in bacteria of different phyla; Appl. Environ. Microbiol. 65 25–35

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hahn M W, Moore E R B and Höfle M G 2000 Role of microcolony formation in the protistan grazing defence of the aquatic bacterium Pseudomonas spp. MWH1; Microb. Ecol. 39 175–185

    PubMed  Google Scholar 

  • Hahn M W and Höfle M G 2001 Grazing of protozoa and its effect on populations of aquatic bacteria; FEMS Microbiol. Ecol. 35 113–121

    Article  CAS  Google Scholar 

  • Henssen D D and Tranvik L J 1998 Aquatic humic substances: ecology and biochemistry (Berlin: Springer)

    Book  Google Scholar 

  • Hobbie J E, Daley R and Jasper S 1977 Use of nucleopore filters for counting bacteria by epifluorescence microscopy; Appl. Environ. Microbiol. 33 1225–1228

    CAS  PubMed  PubMed Central  Google Scholar 

  • Jugnia L B, Tadonléké R D, Simi-Ngando T and Devaux J 2000 The microbial food web in the recently flooded Sep reservoir: diel fluctuations in bacterial biomass and metabolic activity in relation to phytoplankton and flagellate grazers; Microb. Ecol. 40 317–329

    CAS  PubMed  Google Scholar 

  • Kjelleberg S, Hermansson M and Mårdén P 1987 The transient phase between growth and non growth heterotrophic bacteria, with emphasis on the marine environment; Annu. Rev. Microbiol. 41 25–49

    Article  CAS  Google Scholar 

  • Lee W J and Patterson D J 2002 Abundance and biomass of heterotrophic flagellates, and factors controlling their abundance and distribution in sediments of Botany Bay; Microb. Ecol. 43 467–481

    Article  CAS  Google Scholar 

  • Letarte Y and Pinel-Alloul B 1991 Relationships between bacterioplantkon production and limnological variables: necessity of bacterial size considerations; Limnol. Oceanogr. 36 1208–1216

    Article  Google Scholar 

  • May K R 1965 A new graticule for particle counting and sizing; J. Sci. Instrum. 42 500–501

    Article  Google Scholar 

  • Norušis M J 1993 SPSS® for Windows™ base system users guide; release 6.0 (Chicago, USA: SPSS Inc)

    Google Scholar 

  • Overbeck J and Chrost R J 1990 Aquatic microbial ecology: biochemical and molecular approaches (New York: Springer)

    Book  Google Scholar 

  • Pace M L and Cole J J 1996 Regulation of bacteria by resource and predation tested in whole-lake experiments; Limnol. Oceanogr. 41 1448–1460

    Article  CAS  Google Scholar 

  • Rimes C A and Goulder R 1986a Temporal variation in density of epiphytic bacteria on submerged vegetation in a calcareous stream; Lett. Appl. Microbiol. 3 17–21

    Article  Google Scholar 

  • Rimes C A and Goulder R 1986b Suspended bacteria in calcareous and acid headstream: abundance, heterotrophic activity and downstream change; Freshw. Biol. 16 633–651

    Article  Google Scholar 

  • Sanders R W, Porter K G, Bennette S J and Debiase A E 1989 Seasonal pattern of bactivory by flagellates, ciliates, rotifers and cladocerans in a freshwater planktonic community; Limnol. Oceanogr. 34 673–687

    Article  Google Scholar 

  • Schauer M, Kamenik C and Hahn M W 2005 Ecological differentiation within a cosmopolitan group of planktonic freshwater bacteria (SOL cluster, Saprospiraceae, Bacteroidetes); Appl. Environ. Microbiol. 71 5900–5907

    Article  CAS  Google Scholar 

  • Schauer M, Jiang J and Hahn M W 2006 Recurrent seasonal variations in abundance and composition of filamentous SOL cluster bacteria (Saprospiraceae, Bacteroidetes) in oligomesotrophic Lake Mondsee (Austria); Appl. Environ. Microbiol. 72 4704–4712

    Article  CAS  Google Scholar 

  • Servais P and Garnier J 1990 Activité bactérienne hétérotrophe dans la Seine: profils d’incorporation de thymidine et de lucine tritiées; C. R. Acad. Sci. Paris 311(III) 353–360

    Google Scholar 

  • Šimek K and Chrzanowski T H 1992 Direct and indirect evidence of size-selective grazing on pelagic bacteria by freshwater nanoflagellates; Appl. Environ. Microbiol. 58 3715–3720

    PubMed  PubMed Central  Google Scholar 

  • Šimek K, Vrba J, Pernthaler J, Posch T, Hartman P, Nedoma J and Psenner R 1997 Morphological and compositional shifts in an experimental bacterial community influenced by protests with contrasting feeding model; Appl. Environ. Microbiol. 63 587–595

    PubMed  PubMed Central  Google Scholar 

  • Simon M and Wunsch 1998 Temperature control of bacterioplankton growth in a temperate large and deep mesotrophic lake; Aquat. Microb. Ecol. 16 119–130

    Article  Google Scholar 

  • Thouvenot A, Richardot M, Debroas D and Devaux J 1999 Bacterivoir of metazooplankton, ciliates and flagellates in a newly flooded reservoir; J. Plankton Res. 21 1659–1679

    Article  Google Scholar 

  • Tumber V P, Roberts R D, Arts M T, Evans M S and Coldwell De E 1993 The influence of environmental factors on seasonal changes in bacterial cell volume in two prairie saline lakes; Microb. Ecol. 26 9–20

    Article  CAS  Google Scholar 

  • Van Es F B and Meyer-Reil L A 1982 Biomass and metabolic activity of heterotrophic marine bacteria; Adv. Microb. Ecol. 6 111–170

    Google Scholar 

  • Write R T 1988 A model for short-term control of the bacterioplankton by substrate and grazing; Hydrobiologia 159 111–117

    Article  Google Scholar 

  • Wu Q L, Boenigk J and Hahn M W 2004 Successful predation of filamentous bacteria by a nanoflagellate challenges current models of flagellate bacterivory; Appl. Environ. Microbiol. 70 332–339

    Article  CAS  Google Scholar 

  • Yamakanamardi S M 1995 Microbial ecology of three contrasting lowland water courses in Northeast England (England, U K: University of Hull) PhD thesis

    Google Scholar 

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Authors and Affiliations

  1. Aquatic Microbial Ecology Research Laboratory, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore, 570 006, India

    T S Harsha, Sadanand M Yamakanamardi & M Mahadevaswamy

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  1. T S Harsha
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  2. Sadanand M Yamakanamardi
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  3. M Mahadevaswamy
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Correspondence to Sadanand M Yamakanamardi.

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Harsha, T.S., Yamakanamardi, S.M. & Mahadevaswamy, M. Heterotrophic free-living and particle-bound bacterial cell size in the river Cauvery and its downstream tributaries. J Biosci 32, 363–374 (2007). https://doi.org/10.1007/s12038-007-0035-y

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  • DOI: https://doi.org/10.1007/s12038-007-0035-y

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