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Chondroprotective potential of root extracts of Withania somnifera in osteoarthritis

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Abstract

This is the first report describing two novel chondroprotective activities of aqueous extracts of Withania somnifera root powder. First, these extracts had a statistically significant, short-term chondroprotective effect on damaged human osteoarthritic cartilage matrix in 50% of the patients tested. Second, these extracts caused a significant and reproducible inhibition of the gelatinase activity of collagenase type 2 enzyme in vitro.

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Abbreviations

OA:

osteoarthritis

GS:

glucosamine sulphate

PG:

proteoglycan

HPLC:

high performance liquid chromatography

CM:

conditioned media

PG:

proteoglycan

References

  • Clegg D O, Reda D J, Harris C L et al 2006. Glucosamine Chondroitin Arthritis Intervention Trial (GAIT) (National Center for complementary and Alternative Medicine [NCCAM], USA) NCCAM Publication No. D310

    Google Scholar 

  • Dodge G R and Jimenez S A 2003 Glucosamine sulfate modulates the levels of the aggrecan and matrix metalloproteinase-3 synthesized by cultured human osteoarthritis articular chondrocytes; Osteoarthritis Cartilage 11 424–432

    Article  CAS  Google Scholar 

  • Demeule M, Brossard M, Page M, Gingras D and Beliveau R 2000 Matrix metalloproteinase inhibition by green tea catechins; Biochem. Biophys. Acta 1478 51–60

    CAS  PubMed  Google Scholar 

  • Harsulkar A M, Giri A P, Gupta V S, Sainani M N, Deshpande V V, Patankar A G and Ranjekar P K 1998 Characterization of H. armigera gut proteinases and their interaction with proteinase inhibitors using gel X-ray film contact print technique; Electrophoresis 19 1397–1402

    Article  CAS  Google Scholar 

  • Hoemann C D, Sun J, Chrzanowski V and Bhushman MD 2002 A multivalent assay to detect glycosaminoglycan, protein, collagen, RNA, and DNA content in milligram samples of cartilage or hydrogel-based repair cartilage; Anal. Biochem. 300 1–10

    Article  CAS  Google Scholar 

  • Kulkarni R R, Patki P S, Jog V P, Gandage S G and Patwardhan B 1991 Treatment of osteoarthritis with a herbomineral formulation: a double-blind, placebo-controlled, cross-over study; J. Ethnopharmacol. 33 91–95

    Article  CAS  Google Scholar 

  • Mishra L C, Singh B B and Dagenais S 2000 Scientific basis for the therapeutic use of Withania somnifera (ashwagandha): a review; Altern. Med. Rev. 5 334–346

    CAS  PubMed  Google Scholar 

  • Nethery A, Giles I, Jenkins K, Jackson C, Brooks P, Burkhardt D, Ghosh P, Whitelock J, Grady R L and Welgus H G 1992 The chondroprotective drugs, arteparon and sodium pentosan polysulphate, increase collagenase activity and inhibit stomelysin activity in vitro; Biochem. Pharmacol. 44 1549–1553

    Article  CAS  Google Scholar 

  • Outerbridge RE 1961 The etiology of chondromalacia patellae; J. Bone Joint Surg. 43-B 752–757

    Article  CAS  Google Scholar 

  • Richy F, Bruyere O, Ethgen O, Cucherat M, Henrotin Y and Reginster J Y 2003 Structural and symptomatic efficacy of glucosamine and chondroitin in knee osteoarthritis: a comprehensive meta-analysis; Arch. Intern. Med. 163 1514–1522

    Article  CAS  Google Scholar 

  • Smith G N Jr, Yu L P Jr, Brandt K D and Capello W N 1998 Oral administration of doxycycline reduces collagenase and gelatinase activities in extracts of human osteoarthritic cartilage; J. Rheumatol. 25 532–535

    CAS  PubMed  Google Scholar 

  • Tomasz G and Jaworski S 2000 Proteoglycans of human umbilical cord arteries; Acta Biochem. Polonica 47 1081–1091

    Google Scholar 

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Authors and Affiliations

  1. Interactive Research School for Health Affairs (IRSHA), Bhartiya Vidyapeeth Deemed University Medical College Campus, Dhankawadi, Pune, 411043, India

    Venil N Sumantran, Asavari Kulkarni, Sanjay Boddul, Soumya J Koppikar, Abhay Harsulkar & Ulhas V Wagh

  2. Interdisciplinary School of Health Sciences, Pune University, Pune, 411007, India

    Trushna Chinchwade & Bhushan Patwardhan

  3. Center for Rheumatic Diseases, Camp, Pune, 411001, India

    Arvind Chopra (Director)

Authors
  1. Venil N Sumantran
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  2. Asavari Kulkarni
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  3. Sanjay Boddul
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  4. Trushna Chinchwade
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  5. Soumya J Koppikar
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  6. Abhay Harsulkar
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  7. Bhushan Patwardhan
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  8. Arvind Chopra
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  9. Ulhas V Wagh
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Correspondence to Venil N Sumantran.

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Sumantran, V.N., Kulkarni, A., Boddul, S. et al. Chondroprotective potential of root extracts of Withania somnifera in osteoarthritis. J Biosci 32, 299–307 (2007). https://doi.org/10.1007/s12038-007-0030-3

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  • DOI: https://doi.org/10.1007/s12038-007-0030-3

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