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Bifidobacterium breve Probiotic Compared to Lactobacillus casei Causes a Better Reduction in Demyelination and Oxidative Stress in Cuprizone-Induced Demyelination Model of Rat

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Abstract

Despite the anatomical separation, strong evidence suggested a bidirectional association between gut microbiota and central nervous system. Cross-talk between gut microbiota and brain has an important role in the pathophysiology of neurodegenerative disorders and regenerative processes. However, choosing the appropriate probiotics and combination therapy of probiotics to provide a synergistic effect is very crucial. In the present study, we investigated the effect of Lactobacillus casei (L. casei) and Bifidobacterium breve (B. breve) on alternation performance, oxidant/antioxidant biomarkers, the extent of demyelination, and the expression level of HO-1, Nrf-2, Olig2, MBP, PDGFRα, and BDNF in cuprizone (CPZ)-induced demyelination model of rat corpus callosum. In order to induce this model, rats received oral administration of CPZ 0.6% w/w in corn oil for 28 days. Then, L. casei, B. breve, or their combinations were orally administrated for 28 days. Y maze test was performed to investigate the alternation performance. Oxidant/antioxidant biomarkers were determined by colorimetric methods. Extent of demyelination was investigated using FluoroMyelin staining. The genes’ expression levels of antioxidant and myelin lineage cells were assessed by quantitative real time PCR. The results showed the probiotics supplementation significantly improve the alternation performance and antioxidant capacity in demyelinated corpus callosum. Interestingly, B. breve supplementation alleviated demyelination and oxidative stress levels more than the administration of L. casei alone or the combination of two probiotics. These observations suggest that B. breve could provide a supplementary strategy for the treatment of multiple sclerosis by increasing antioxidant capacity and remyelination.

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Data Availability

The datasets analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to acknowledge the kind assistance of Mr. Moein Shirzad.

Funding

This study was performed as a part of Master of Science thesis and has been financially supported by Babol University of Medical Sciences, Babol, Iran (Grant No. 724133976).

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Authors and Affiliations

  1. Student Research Committee, Babol University of Medical Sciences, Babol, Iran

    Nima Hasaniani

  2. Department of Clinical Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran

    Nima Hasaniani & Sahar Rostami-Mansoor

  3. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

    Maryam Ghasemi-Kasman

  4. Department of Physiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran

    Maryam Ghasemi-Kasman

  5. Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran

    Mehrdad Halaji

  6. Department of Laboratory Sciences, Faculty of Paramedical Sciences, Babol University of Medical Sciences, Babol, Iran

    Sahar Rostami-Mansoor

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Contributions

Conceptualization: Sahar Rostami-Mansoor; Nima Hasaniani; methodology: Nima Hasaniani, Sahar Rostami-Mansoor, Maryam Ghasemi-Kasman; Mehrdad Halaji; formal analysis and investigation: Sahar Rostami-Mansoor; Maryam Ghasemi-Kasman; writing original draft preparation: Sahar Rostami-Mansoor; editing: Maryam Ghasemi-Kasman, Mehrdad Halaji; supervision: Sahar Rostami-Mansoor.

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Correspondence to Sahar Rostami-Mansoor.

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This study was approved by the ethical committee of Babol University of Medical Sciences (Approval No.: IR.MUBABOL.AEC.1401.005).

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Highlights

• L. casei and B. breve supplementation improves working memory in CPZ-induced demyelination model.

• L. casei and B. breve supplementation decreases oxidative stress in the brain of CPZ receiving rats.

• L. casei and B. breve supplementation decreases the extent of demyelination in the corpus callosum of CPZ-receiving rats.

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Hasaniani, N., Ghasemi-Kasman, M., Halaji, M. et al. Bifidobacterium breve Probiotic Compared to Lactobacillus casei Causes a Better Reduction in Demyelination and Oxidative Stress in Cuprizone-Induced Demyelination Model of Rat. Mol Neurobiol 61, 498–509 (2024). https://doi.org/10.1007/s12035-023-03593-4

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