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Molecular Neurobiology

, Volume 56, Issue 1, pp 748–760 | Cite as

Stem Cells from Human Exfoliated Deciduous Teeth Modulate Early Astrocyte Response after Spinal Cord Contusion

  • Fabrício Nicola
  • Marília Rossato Marques
  • Felipe Odorcyk
  • Letícia Petenuzzo
  • Dirceu Aristimunha
  • Adriana Vizuete
  • Eduardo Farias Sanches
  • Daniela Pavulack Pereira
  • Natasha Maurmann
  • Carlos-Alberto Gonçalves
  • Patricia Pranke
  • Carlos Alexandre Netto
Article
  • 168 Downloads

Abstract

The transplantation of stem cells from human exfoliated deciduous teeth (SHED) has been studied as a possible treatment strategy for spinal cord injuries (SCIs) due to its potential for promoting tissue protection and functional recovery. The aim of the present study was to investigate the effects of the early transplantation of SHED on glial scar formation and astrocytic reaction after an experimental model of SCI. Wistar rats were spinalized using the NYU Impactor. Animals were randomly distributed into three groups: control (naive) (animal with no manipulation); SCI (receiving laminectomy followed by SCI and treated with vehicle), and SHED (SCI rat treated with intraspinal SHED transplantation, 1 h after SCI). In vitro investigation demonstrated that SHED were able to express mesenchymal stem cells, vimentin and S100B markers, related with neural progenitor and glial cells, respectively. The acute SHED transplantation promoted functional recovery, measured as from the first week after spinal cord contusion by Basso, Beattie, and Bresnahan scale. Twenty-four and 48 h after lesion, flow cytometry revealed a spinal cord vimentin+ cells increment in the SHED group. The increase of vimentin+ cells was confirmed by immunofluorescence. Moreover, the bioavailability of astrocytic proteins such as S100B and Kir4.1 shown to be increased in the spinal cord of SHED group, whereas there was a glial scar reduction, as indicated by ELISA and Western blot techniques. The presented results support that SHED act as a neuroprotector agent after transplantation, probably through paracrine signaling to reduce glial scar formation, inducing tissue plasticity and functional recovery.

Keywords

Spinal cord injury Human dental pulp stem cells Glial scar formation Progenitor cells 

Abbreviations

APC

Allophycocyanin

AQP4

Aquaporin 4

BBB

Basso, Beattie, and Bresnahan scale

FITC

Fluorescein isothiocyanate

GFAP

Glial fibrillary acidic protein

Kir

Inward rectifying potassium channel

MASCIS

Multicenter Animal Spinal Cord Injury Study

MSCs

Mesenchymal stem cells

PE

Phycoerythrin

PMSF

Phenylmethyl-sulphonyl fluoride

SCI

Spinal cord injury

SHED

Stem cells from human exfoliated deciduous teeth

S100B

Calcium-binding protein

TBS

Tris-buffered saline

Tx

Triton X-100

Vimentin

Progenitor neural cells

βIII-tubulin

Neuronal microtubule protein

Notes

Acknowledgements

This work was supported by funds from the Conselho Nacional de Desenvolvimento Cientifico e Tecnológico do Brasil (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Stem Cell Research Institute.

Compliance with Ethical Standards

All procedures were in accordance with the Guide for the Care and Use of Laboratory Animals adopted by the National Institute of Health (USA) and with the Federation of Brazilian Societies for Experimental Biology and with the Brazilian Law for Laboratory Animals care no 11.794. The experimental study was approved by the Research Ethics Committee of the University (#26116). The procedures for obtaining and isolate the SHED were approved by the Ethics Committee of the Universidade Federal do Rio Grande do Sul (#296/08).

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Fabrício Nicola
    • 1
    • 2
  • Marília Rossato Marques
    • 1
    • 2
  • Felipe Odorcyk
    • 1
    • 2
  • Letícia Petenuzzo
    • 2
  • Dirceu Aristimunha
    • 2
  • Adriana Vizuete
    • 2
  • Eduardo Farias Sanches
    • 2
  • Daniela Pavulack Pereira
    • 3
  • Natasha Maurmann
    • 3
    • 4
  • Carlos-Alberto Gonçalves
    • 2
  • Patricia Pranke
    • 3
    • 4
    • 5
  • Carlos Alexandre Netto
    • 2
  1. 1.Post Graduate Program in NeuroscienceUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Department of Biochemistry, Institute of Basic Health SciencesUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  3. 3.Hematology and Stem Cell LaboratoryUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  4. 4.Post Graduate Program in PhysiologyUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  5. 5.Stem Cell Research InstitutePorto AlegreBrazil

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