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Arbutus andrachne L. Reverses Sleep Deprivation-Induced Memory Impairments in Rats

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Abstract

Sleep deprivation (SD) is associated with cognitive deficits. It was found to affect the hippocampus region of the brain by impairing memory formation. This impairment is suggested to be caused by elevation in oxidative stress in the body, including the brain during SD. It was hypothesized that the methanolic extract of the fruits of Arbutus andrachne L. (Ericaceae) will prevent chronic SD-induced impairment of hippocampal memory via its antioxidative properties. The methanolic extract of the fruits of A. andrachne was evaluated for its beneficial properties to reverse SD-induced cognitive impairment in rats. Animals were sleep deprived for 8 weeks using a multiple platform model. The extract was administered i.p. at three doses (50, 200, and 500 mg/kg). Behavioral studies were conducted to test the spatial learning and memory using radial arm water maze (RAWM). In addition, the hippocampus was dissected to analyze the following oxidative stress markers: glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG, glutathione peroxidase (GPx), and catalase. Chronic SD impaired short- and long-term memories (P < 0.05). Treatment of animals with A. andrachne fruit extract at all doses prevented long-term memory impairment induced by SD while such treatment prevented short-term memory impairment only at 200 and 500 mg/kg dose levels. Moreover, A. andrachne fruit extract normalized the reduction in the hippocampus GSH/GSSG ratio and activity of GPx, and catalase (P < 0.05) induced by chronic sleep deprivation. Chronic sleep deprivation impaired both short- and long-term memory formation, while methanolic extract of A. andrachne fruits reversed this impairment, probably through normalizing oxidative stress in the hippocampus.

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Abbreviations

REM:

Rapid eye movement

NREM:

Non-rapid eye movement

SD:

Sleep deprivation

RAWM:

Radial arm water maze

WPF:

Wide platform

GSH:

Glutathione

GSSG:

Oxidized glutathione

GPx:

Glutathione peroxidase

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Acknowledgements

This research was supported by the Deanship of Research (project number 99/2011), Jordan University of Science and Technology, Irbid, Jordan.

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Authors and Affiliations

  1. Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Karem H. Alzoubi

  2. Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Bayan S. Malkawi & Tamam El-Elimat

  3. Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Omar F. Khabour

  4. College of Pharmacy, Qatar University, Doha, 2713, Qatar

    Feras Q. Alali

Authors
  1. Karem H. Alzoubi
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  2. Bayan S. Malkawi
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  3. Omar F. Khabour
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  4. Tamam El-Elimat
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  5. Feras Q. Alali
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Corresponding author

Correspondence to Feras Q. Alali.

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The study protocol was approved by the Animal Care and Use Committee (ACUC) at Jordan University of Science and Technology. All study procedures were done in accordance with the National Institutes of Health guide for the care and use of laboratory animals.

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Dr Feras Alali is officially on leave from Jordan University of Science and Technology.

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Alzoubi, K.H., Malkawi, B.S., Khabour, O.F. et al. Arbutus andrachne L. Reverses Sleep Deprivation-Induced Memory Impairments in Rats. Mol Neurobiol 55, 1150–1156 (2018). https://doi.org/10.1007/s12035-017-0387-8

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  • DOI: https://doi.org/10.1007/s12035-017-0387-8

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