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Molecular Neurobiology

, Volume 54, Issue 5, pp 3652–3664 | Cite as

Delayed Treatment with Green Tea Polyphenol EGCG Promotes Neurogenesis After Ischemic Stroke in Adult Mice

  • Jian-Cheng Zhang
  • Hang Xu
  • Yin Yuan
  • Jia-Yi Chen
  • Yu-Jing Zhang
  • Yun LinEmail author
  • Shi-Ying YuanEmail author
Article

Abstract

(−)-Epigallocatechin-3‑gallate (EGCG), the predominant constituent of green tea, has been demonstrated to be neuroprotective against acute ischemic stroke. However, the long-term actions of EGCG on neurogenesis and functional recovery after ischemic stroke have not been identified. In this study, C57BL/6 mice underwent middle cerebral artery occlusion (60 min) followed by reperfusion for 28 days. Neural progenitor cells (NPCs) were isolated from ipsilateral subventricular zone (SVZ) at 14 days post-ischemia (dpi). The effects of EGCG on the proliferation and differentiation of NPCs were examined in vivo and in vitro. Behavioral assessments were made 3 days before MCAO and at 28 dpi. SVZ NPCs were stimulated with lipopolysaccharide (LPS) in vitro to mimic the inflammatory response after ischemic stroke. We found that 14 days treatment with EGCG significantly increased the proliferation of SVZ NPCs and the migration of SVZ neuroblasts, as well as functional recovery, perhaps through M2 phenotype induction in microglia. LPS stimulation promoted the neuronal differentiation in cultured NPCs from the ischemic SVZ. EGCG treatment (20 or 40 μM) further significantly increased the neuronal differentiation of LPS-stimulated SVZ NPCs. After screening for multiple signaling pathways, the AKT signaling pathway was found to be involved in EGCG-mediated proliferation and neuronal differentiation of NPCs in vitro. Taken together, our results reveal a previously uncharacterized role of EGCG in the augment of proliferation and neuronal differentiation of SVZ NPCs and subsequent spontaneous recovery after ischemic stroke. Thus, the beneficial effects of EGCG on neurogenesis and stroke recovery should be considered in developing therapeutic approaches.

Keywords

(−)-Epigallocatechin-3‑gallate Ischemic stroke Neurogenesis Neural progenitors Recovery 

Notes

Authors’ Contributions

SYY, YL, and JCZ contributed in the experimental design. JCZ, HX, and YY contributed in the stroke model, functional assays, and Western blotting. JCZ, YY, JYC, and YJZ helped in the immunocytochemistry and functional assays. JCZ helped in the neurosphere cultures. JCZ contributed in the imaging tools. SYY, YL, and JCZ contributed in the data analysis. JCZ wrote the article

Compliance with Ethical Standards

Sources of Funding

This work was supported by the Huazhong University of Science and Technology Innovation fund (0118530238) and the National Natural Science Foundation of China (Grant Nos. 81571138, 81571286).

Disclosures

None.

References

  1. 1.
    Donnan GA, Fisher M, Macleod M, Davis SM (2008) Stroke. Lancet 371:1612–1623CrossRefPubMedGoogle Scholar
  2. 2.
    Go AS, Mozaffarian D, Roger VL, Benjamin EJ, Berry JD, Blaha MJ, Dai S, Ford ES et al (2014) Heart disease and stroke statistics—2014 update: a report from the American Heart Association. Circulation 129:e28–e292CrossRefPubMedGoogle Scholar
  3. 3.
    Schwamm LH, Ali SF, Reeves MJ, Smith EE, Saver JL, Messe S, Bhatt DL, Grau-Sepulveda MV et al (2013) Temporal trends in patient characteristics and treatment with intravenous thrombolysis among acute ischemic stroke patients at Get With The Guidelines-Stroke hospitals. Circ Cardiovasc Qual Outcomes 6:543–549CrossRefPubMedGoogle Scholar
  4. 4.
    Suzuki Y, Miyoshi N, Isemura M (2012) Health-promoting effects of green tea. Proc Jpn Acad Ser B Phys Biol Sci 88:88–101CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Graham HN (1992) Green tea composition, consumption, and polyphenol chemistry. Prev Med 21:334–350CrossRefPubMedGoogle Scholar
  6. 6.
    Singh BN, Shankar S, Srivastava RK (2011) Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochem Pharmacol 82:1807–1821CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Afzal M, Safer AM, Menon M (2015) Green tea polyphenols and their potential role in health and disease. Inflammopharmacology 23:151–161CrossRefPubMedGoogle Scholar
  8. 8.
    Yao C, Zhang J, Liu G, Chen F, Lin Y (2014) Neuroprotection by (−)-epigallocatechin-3-gallate in a rat model of stroke is mediated through inhibition of endoplasmic reticulum stress. Mol Med Rep 9:69–76PubMedGoogle Scholar
  9. 9.
    Zhang F, Li N, Jiang L, Chen L, Huang M (2015) Neuroprotective effects of (−)-epigallocatechin-3-gallate against focal cerebral ischemia/reperfusion injury in rats through attenuation of inflammation. Neurochem Res 40:1691–1698CrossRefPubMedGoogle Scholar
  10. 10.
    Xie W, Ramakrishna N, Wieraszko A, Hwang YW (2008) Promotion of neuronal plasticity by (−)-epigallocatechin-3-gallate. Neurochem Res 33:776–783CrossRefPubMedGoogle Scholar
  11. 11.
    van Praag H, Lucero MJ, Yeo GW, Stecker K, Heivand N, Zhao C, Yip E, Afanador M et al (2007) Plant-derived flavanol (−)epicatechin enhances angiogenesis and retention of spatial memory in mice. J Neurosci 27:5869–5878CrossRefPubMedGoogle Scholar
  12. 12.
    Wang Y, Li M, Xu X, Song M, Tao H, Bai Y (2012) Green tea epigallocatechin-3-gallate (EGCG) promotes neural progenitor cell proliferation and sonic hedgehog pathway activation during adult hippocampal neurogenesis. Mol Nutr Food Res 56:1292–1303CrossRefPubMedGoogle Scholar
  13. 13.
    Itoh T, Imano M, Nishida S, Tsubaki M, Mizuguchi N, Hashimoto S, Ito A, Satou T (2012) (−)-Epigallocatechin-3-gallate increases the number of neural stem cells around the damaged area after rat traumatic brain injury. J Neural Transm (Vienna) 119:877–890CrossRefGoogle Scholar
  14. 14.
    Zhang J, Mao X, Zhou T, Cheng X, Lin Y (2014) IL-17A contributes to brain ischemia reperfusion injury through calpain-TRPC6 pathway in mice. Neuroscience 274:419–428CrossRefPubMedGoogle Scholar
  15. 15.
    Zhang J, Wu Y, Weng Z, Zhou T, Feng T, Lin Y (2014) Glycyrrhizin protects brain against ischemia-reperfusion injury in mice through HMGB1-TLR4-IL-17A signaling pathway. Brain Res 1582:176–186CrossRefPubMedGoogle Scholar
  16. 16.
    Sun F, Wang X, Mao X, Xie L, Jin K (2012) Ablation of neurogenesis attenuates recovery of motor function after focal cerebral ischemia in middle-aged mice. PLoS One 7:e46326CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Gertz K, Kronenberg G, Kalin RE, Baldinger T, Werner C, Balkaya M, Eom GD, Hellmann-Regen J et al (2012) Essential role of interleukin-6 in post-stroke angiogenesis. Brain 135:1964–1980CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Song J, Cho KJ, Cheon SY, Kim SH, Park KA, Lee WT, Lee JE (2013) Apoptosis signal-regulating kinase 1 (ASK1) is linked to neural stem cell differentiation after ischemic brain injury. Exp Mol Med 45:e69CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Shin JA, Lim SM, Jeong SI, Kang JL, Park EM (2014) Noggin improves ischemic brain tissue repair and promotes alternative activation of microglia in mice. Brain Behav Immun 40:143–154CrossRefPubMedGoogle Scholar
  20. 20.
    Ransohoff RM, Brown MA (2012) Innate immunity in the central nervous system. J Clin Invest 122:1164–1171CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Thored P, Heldmann U, Gomes-Leal W, Gisler R, Darsalia V, Taneera J, Nygren JM, Jacobsen SE et al (2009) Long-term accumulation of microglia with proneurogenic phenotype concomitant with persistent neurogenesis in adult subventricular zone after stroke. Glia 57:835–849CrossRefPubMedGoogle Scholar
  22. 22.
    Hu X, Li P, Guo Y, Wang H, Leak RK, Chen S, Gao Y, Chen J (2012) Microglia/macrophage polarization dynamics reveal novel mechanism of injury expansion after focal cerebral ischemia. Stroke 43:3063–3070CrossRefPubMedGoogle Scholar
  23. 23.
    Brifault C, Gras M, Liot D, May V, Vaudry D, Wurtz O (2015) Delayed pituitary adenylate cyclase-activating polypeptide delivery after brain stroke improves functional recovery by inducing m2 microglia/macrophage polarization. Stroke 46:520–528CrossRefPubMedGoogle Scholar
  24. 24.
    Xu D, Zhang F, Wang Y, Sun Y, Xu Z (2014) Microcephaly-associated protein WDR62 regulates neurogenesis through JNK1 in the developing neocortex. Cell Rep 6:104–116CrossRefPubMedGoogle Scholar
  25. 25.
    Zhang D, Guo M, Zhang W, Lu XY (2011) Adiponectin stimulates proliferation of adult hippocampal neural stem/progenitor cells through activation of p38 mitogen-activated protein kinase (p38MAPK)/glycogen synthase kinase 3beta (GSK-3beta)/beta-catenin signaling cascade. J Biol Chem 286:44913–44920CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Zhang C, Chopp M, Cui Y, Wang L, Zhang R, Zhang L, Lu M, Szalad A et al (2010) Cerebrolysin enhances neurogenesis in the ischemic brain and improves functional outcome after stroke. J Neurosci Res 88:3275–3281CrossRefPubMedPubMedCentralGoogle Scholar
  27. 27.
    Rosa AI, Goncalves J, Cortes L, Bernardino L, Malva JO, Agasse F (2010) The angiogenic factor angiopoietin-1 is a proneurogenic peptide on subventricular zone stem/progenitor cells. J Neurosci 30:4573–4584CrossRefPubMedGoogle Scholar
  28. 28.
    Nicoleau C, Benzakour O, Agasse F, Thiriet N, Petit J, Prestoz L, Roger M, Jaber M et al (2009) Endogenous hepatocyte growth factor is a niche signal for subventricular zone neural stem cell amplification and self-renewal. Stem Cells 27:408–419CrossRefPubMedGoogle Scholar
  29. 29.
    Arvidsson A, Collin T, Kirik D, Kokaia Z, Lindvall O (2002) Neuronal replacement from endogenous precursors in the adult brain after stroke. Nat Med 8:963–970CrossRefPubMedGoogle Scholar
  30. 30.
    Parent JM, Vexler ZS, Gong C, Derugin N, Ferriero DM (2002) Rat forebrain neurogenesis and striatal neuron replacement after focal stroke. Ann Neurol 52:802–813CrossRefPubMedGoogle Scholar
  31. 31.
    Thored P, Arvidsson A, Cacci E, Ahlenius H, Kallur T, Darsalia V, Ekdahl CT, Kokaia Z et al (2006) Persistent production of neurons from adult brain stem cells during recovery after stroke. Stem Cells 24:739–747CrossRefPubMedGoogle Scholar
  32. 32.
    Chen J, Zhang ZG, Li Y, Wang Y, Wang L, Jiang H, Zhang C, Lu M et al (2003) Statins induce angiogenesis, neurogenesis, and synaptogenesis after stroke. Ann Neurol 53:743–751CrossRefPubMedGoogle Scholar
  33. 33.
    Shioda N, Han F, Morioka M, Fukunaga K (2008) Bis(1-oxy-2-pyridinethiolato)oxovanadium(IV) enhances neurogenesis via phosphatidylinositol 3-kinase/Akt and extracellular signal regulated kinase activation in the hippocampal subgranular zone after mouse focal cerebral ischemia. Neuroscience 155:876–887CrossRefPubMedGoogle Scholar
  34. 34.
    Fan W, Dai Y, Xu H, Zhu X, Cai P, Wang L, Sun C, Hu C et al (2014) Caspase-3 modulates regenerative response after stroke. Stem Cells 32:473–486CrossRefPubMedGoogle Scholar
  35. 35.
    Gregorian C, Nakashima J, Le Belle J, Ohab J, Kim R, Liu A, Smith KB, Groszer M et al (2009) Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesis. J Neurosci 29:1874–1886CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Critical Care Medicine, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Anesthesia & Critical Care Medicine, Institute of Anesthesia and Critical Care Medicine, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  3. 3.Department of Critical Care Medicine, First Affiliated Hospital of the Medical CollegeShihezi UniversityShihezi CityChina

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