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Monocarboxylate Transporter 1 in the Medial Prefrontal Cortex Developmentally Expresses in Oligodendrocytes and Associates with Neuronal Amounts

Molecular Neurobiology volume 54, pages 2315–2326 (2017)Cite this article

Abstract

Lactate is an energy substrate in adult brain especially when glucose is withdrawn or only lactate is present as main energy source. Besides, the most abundant lactate transporter in brain—monocarboxylate transporter 1 (MCT1)—was recognized recently. Despite this, MCT1 expressions in central nervous system (CNS) have not been clearly understood. Medial prefrontal cortex (mPFC), taking part in many higher executive functions in brain, is chosen here for observing MCT1 expressions in mice in 12 months. As results showed, MCT1 is gradually increased from an initial level at the 1st week to a high level at the 6th week and then gradually decreased to a low level at the 12th month. Besides, neuronal amounts change in a similar trend as MCT1 that neurons at the 6th week are more than that of at the 1st week and the 12th month. Also, MCT1 expressions are highly correlated with neuronal amounts, while MCT1 does not localize within neurons, instead localize around axons. On the other hand, MCT1 does localize to oligodendrocytes (OLs) without localizing to other glial cells (astrocytes and microglias). Importantly, the amounts of OLs change in a similar trend as MCT1, while the amounts of other glial cells do not change obviously in the mPFC in vivo in 12 months. These results demonstrate that the changeable expressions of MCT1 in the mPFC in vivo in 12 months may be mainly contributed by OLs and associate with the neuronal amounts. Above all, it infers that in vivo, MCT1 which is changeably expressed in OLs may further affect neuronal amounts in the mPFC in 12 months.

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Abbreviations

ANOVAs:

Two-way analyses of variance

BSA:

Bovine serum albumin

CNS:

Central nervous system

DAPI:

4,6-Diamidino-2-phenylindole

FITC:

Fluorescein isothiocyanate

GFAP:

Glial fibrillary acidic protein

HRP:

Horseradish peroxidase

MBP:

Myelin basic protein

MCT1:

Monocarboxylate transporter 1

mPFC:

Medial prefrontal cortex

Neu N:

Neuronal nuclei

OD:

Optical density

OLs:

Oligodendrocytes

OX42:

Anti-CD11b/c antibody

PBS:

Phosphate-buffered saline

PFA:

Paraformaldehyde

RIPA:

Radio-immunoprecipitation assay

SD:

Standard deviation

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SMI32:

Non-phosphorylated neurofilament

TRITC:

Tetramethyl rhodamine isothiocyanate

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (Grant No. 31371147) and the High Education Teaching Reform Project of Chongqing (No. 132080).

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Affiliations

  1. Department of Physiology, Third Military Medical University, 30 Gaotanyan Main Street, Shapingba District, Chongqing, 400038, China

    Mao Zhang & Zhongxiang Yao

  2. Battalion 14 of Cadet Brigade, Third Military Medical University, 30 Gaotanyan Main Street, Shapingba District, Chongqing, 400038, China

    Ziyi Ma

  3. Battalion 10 of Cadet Brigade, Third Military Medical University, 30 Gaotanyan Main Street, Shapingba District, Chongqing, 400038, China

    Haochen Qin

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  1. Mao Zhang
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Correspondence to Zhongxiang Yao.

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Statement on Welfare of Animals

All procedures were carried out in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 80–23) revised 1996. All animal experiments were approved by the Third Military Medical University Committee on the Care and Use of Laboratory Animals. This article does not contain any studies with human participants performed by any of the authors.

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figure 8

The change of MCT1+ cells in the mPFC in vivo from the 1st week to the 8th week. As arrowheads pointed, MCT1+ cells equally distribute in the mPFC in vivo, showing round and regular morphology and mainly localize to cytoplasm and cell membrane, from the 1st week to the 8th week. MCT1 expressions are gradually increased from the 1st week to the 3rd week and keep at high levels among the 3rd week and the 6th week. At the 7th and the 8th week, MCT1 is decreased to the levels similar to that of the 1st week and the 2nd week. Scale bars, 100 and 25 μm. (GIF 453 kb)

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figure 9

The change of MCT1+ cells in the mPFC in vivo from the 1st month to the 12th month. As arrowheads showed, MCT1+ cells uniformly distribute in the mPFC in vivo, localizing regularly to cytoplasm and cell membrane. From the 1st month to the 7th month, no significant differences are found in MCT1 expressions. From the 8th month to the 12th month, MCT1 expressions are gradually decreased to a level lower than that of the 1st month. Scale bars, 100 and 25 μm. (GIF 304 kb)

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Zhang, M., Ma, Z., Qin, H. et al. Monocarboxylate Transporter 1 in the Medial Prefrontal Cortex Developmentally Expresses in Oligodendrocytes and Associates with Neuronal Amounts. Mol Neurobiol 54, 2315–2326 (2017). https://doi.org/10.1007/s12035-016-9820-7

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Keywords

  • Monocarboxylate transporter 1 (MCT1)
  • Medial prefrontal cortex (mPFC)
  • Oligodendrocytes (OLs)
  • Neurons
  • Development