Molecular Neurobiology

, Volume 54, Issue 1, pp 748–754

Pramlintide Antagonizes Beta Amyloid (Aβ)- and Human Amylin-Induced Depression of Hippocampal Long-Term Potentiation

  • R. Kimura
  • D. MacTavish
  • J. Yang
  • D. Westaway
  • Jack H. Jhamandas
Article

DOI: 10.1007/s12035-016-9684-x

Cite this article as:
Kimura, R., MacTavish, D., Yang, J. et al. Mol Neurobiol (2017) 54: 748. doi:10.1007/s12035-016-9684-x

Abstract

Accumulation of amyloid-β peptide (Aβ) is a pathological hallmark of Alzheimer’s disease (AD). We have previously demonstrated that electrophysiological and neurotoxic effects of Aβ and human amylin are expressed via the amylin receptor. Recently, pramlintide, a synthetic analog of amylin, has been reported to improve cognitive function in transgenic AD mouse models. In this study, we examined the effects of pramlintide on Aβ1–42 and human amylin-evoked depression of long-term potentiation (LTP) at Schaeffer collateral-CA1 hippocampal synapses. In mouse hippocampal brain slices, field excitatory postsynaptic potentials (fEPSPs) were recorded from the stratum radiatum layer of the CA1 area in response to electrical stimulation of Schaeffer collateral afferents and LTP induced by 3-theta-burst stimulation (TBS) protocol. Aβ1–42 (50 nM) and human amylin (50 nM), but not Aβ42–1 (50 nM), depressed LTP. Pre-application of pramlintide (250 nM) blocked Aβ- and human amylin-induced reduction of LTP without affecting baseline transmission or LTP. We also examined the effects of pramlintide on LTP in transgenic mice (TgCRND8) that over-express amyloid precursor protein. In contrast to wild-type controls, where robust LTP was observed, 10- to 12-month-old TgCRND8 mice show blunted LTP. In TgCRND8 mice, basal LTP is enhanced by application of pramlintide. Our data indicate that pramlintide acts as a functional amylin receptor antagonist to reverse the effects of Aβ1–42 and human amylin on LTP and also increases LTP in transgenic mice that demonstrate increased ambient brain amyloid levels. Amylin receptor antagonists may thus serve as potentially useful therapeutic agents in treatment of AD.

Keywords

Alzheimer’s disease Diabetes Hippocampus LTP Amyloid Amylin receptor Synaptic plasticity 

Funding information

Funder NameGrant NumberFunding Note
Canadian Institutes of Health Research
  • MOP93601
Alberta Prion Research Institute
  • APRI 201400009
Alberta Innovates - Health Solutions
  • Visiting Scientist award 201401129

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • R. Kimura
    • 1
    • 2
  • D. MacTavish
    • 2
    • 4
  • J. Yang
    • 2
    • 4
    • 5
  • D. Westaway
    • 2
    • 3
    • 4
    • 5
  • Jack H. Jhamandas
    • 2
    • 4
  1. 1.Faculty of EngineeringTokyo University of ScienceSanyo-onodaJapan
  2. 2.Department of Medicine (Neurology)University of AlbertaEdmontonCanada
  3. 3.Department of BiochemistryUniversity of AlbertaEdmontonCanada
  4. 4.Neuroscience and Mental Health InstituteUniversity of AlbertaEdmontonCanada
  5. 5.Centre for Prions and Protein Folding DiseasesUniversity of AlbertaEdmontonCanada

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