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Heterogeneity of Brain Ribosomal Genes Expression Following Positive Fighting Experience in Male Mice as Revealed by RNA-Seq

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Abstract

Repeated positive fighting experience in daily agonistic interactions is accompanied by changes of brain neurotransmitter activity and genes’ expression in male mice. This paper is focused on the analysis of ribosomal genes expression data as revealed by whole-transcriptome analysis (RNA-Seq) in five brain regions of male mice with long repeated experience of aggression accompanied by wins (winners). Downregulation of most Rps, Rpl, Mrps, and Mrpl genes was found in the midbrain raphe nuclei and striatum and upregulation—in the hippocampus and hypothalamus of the winners. There were no changes in ribosomal gene expression in the ventral tegmental area. The data allow considering the alteration in ribosomal gene expression as an animal model of ribosomal dysfunction developed under positive fighting experience in male mice.

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Acknowledgements

This work was supported by Russian Science Foundation (project nos. 14-15-00063 and 14-14-00269 for behavioral experiments, sequencing, and bioinformatics).

Author Contributions

D.A.S., I.L.K., and A.G.G. contributed substantially to behavioral data acquisition, received brain materials; V.N.B. implemented bioinformatics assessing of RNA-Seq data, analyzed and interpreted data, and wrote the main manuscript text. NNK performed study design, analyzed the RNA-seq database and interpreted data, wrote the main manuscript text. Y. L.O. revised critically. All authors gave final approval.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, pr. Ac. Lavrentyeva, 10, Novosibirsk, 630090, Russia

    Dmitry A Smagin, Irina L Kovalenko, Anna G Galyamina, Yuriy L Orlov, Vladimir N Babenko & Natalia N Kudryavtseva

  2. Novosibirsk State University, Novosibirsk, 630090, Russia

    Yuriy L Orlov, Vladimir N Babenko & Natalia N Kudryavtseva

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  1. Dmitry A Smagin
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Correspondence to Vladimir N Babenko or Natalia N Kudryavtseva.

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Russian Science Foundation, grant nos. 14-15-00063 and 14-14-00269

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None of the authors have any conflicts of interest to report.

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Smagin, D.A., Kovalenko, I.L., Galyamina, A.G. et al. Heterogeneity of Brain Ribosomal Genes Expression Following Positive Fighting Experience in Male Mice as Revealed by RNA-Seq. Mol Neurobiol 55, 390–401 (2018). https://doi.org/10.1007/s12035-016-0327-z

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