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Neurochemical properties of the synapses between the parabrachial nucleus-derived CGRP-positive axonal terminals and the GABAergic neurons in the lateral capsular division of central nucleus of amygdala

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Abstract

The lateral capsular division of central nucleus of amygdala (CeC) contains neurons using γ-amino butyric acid (GABA) as the predominant neurotransmitter and expresses abundant calcitonin gene-related peptide (CGRP)-positive terminals. However, the relationship between them has not been revealed yet. Using GAD67-green fluorescent protein (GFP) knock-in mouse, we investigated the neurochemical features of synapses between CGRP-positive terminals and GABAergic neurons within CeC and the potential involvement of CGRP1 receptor by combining fluorescent in situ hybridization for CGRP1 receptor mRNA with immunofluorescent histochemistry for GFP and CGRP. The ultrastructures of these synapses were investigated with pre-embedding electron microscopy for GFP and CGRP. We found that some GABAergic neurons in the CeC received parabrachial nucleus (PBN) derived CGRP innervations and some of these GABAergic neurons can be activated by subcutaneous injection of formalin. Moreover, more than 90 % GABAergic neurons innervated by CGRP-positive terminal also express CGRP1 receptor mRNA. The CGRP-positive fibers made symmetric synapses onto the GABAergic somata, and asymmetric synapses onto the GABA-LI dendritic shafts and spines. This study provides direct ultrastructural evidences for the synaptic contacts between CGRP-positive terminals and GABAergic neurons within the CeC, which may underlie the pain-related neural pathway from PBN to CeC and be involved in the chronic pain modulation.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (No. 81171050 to Y.-L. Dong) and International Science & Technology Cooperation Program of China (No. 2011DFA32560 to S.-X. Wu).

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Authors and Affiliations

  1. Department of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi’an, 710032, People’s Republic of China

    Ya-Cheng Lu, Yan-Zhou Chen, Yan-Yan Wei, Xiao-Tao He, Xia Li, Wei Hu, Wen Wang, Sheng-Xi Wu & Yu-Lin Dong

  2. School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China

    Yan-Zhou Chen, Xiao-Tao He & Wei Hu

  3. Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Japan

    Yuchio Yanagawa

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  1. Ya-Cheng Lu
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Correspondence to Wen Wang, Sheng-Xi Wu or Yu-Lin Dong.

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Ya-Cheng Lu and Yan-Zhou Chen contributed equally to this work.

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Supplementary Fig. 1

Graphic illustration showing the boundaries of different subnuclei of CeA. A: Nissl staining indicating the localization of central amygdaloid nucleus. B: high-magnification image of boxed area in A showing the different subnuclei of CeA; C: GABAergic neuron distribution in CeA in same section as A and B. *: one vessel signal in bright and dark field photos. Scale bars = 1 mm in A, 200 μm in B, 100 μm in C. (TIFF 1722 kb)

Supplementary Fig. 2

Immunofluorescent images showing the double-labeling of GFP-LI neurons (green, A) and NeuN (red, B). Scale bars = 100 μm. (TIFF 3521 kb)

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Lu, YC., Chen, YZ., Wei, YY. et al. Neurochemical properties of the synapses between the parabrachial nucleus-derived CGRP-positive axonal terminals and the GABAergic neurons in the lateral capsular division of central nucleus of amygdala. Mol Neurobiol 51, 105–118 (2015). https://doi.org/10.1007/s12035-014-8713-x

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