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SNAP25 Ameliorates Sensory Deficit in Rats with Spinal Cord Transection

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Abstract

Spinal cord injury causes sensory loss below the level of lesion. Synaptosomal-associated protein 25 (SNAP25) is a t-SNARE protein essential for exocytosis and neurotransmitter release, but its role in sensory functional recovery has not been determined. The aim of the present study is therefore to investigate whether SNAP25 can promote sensory recovery. By 2D proteomics, we found a downregulation of SNAP25 and then constructed two lentiviral vectors, Lv-exSNAP25 and Lv-shSNAP25, which allows efficient and stable RNAi-mediated silencing of endogenous SNAP25. Overexpression of SNAP25 enhanced neurite outgrowth in vitro and behavior response to thermal and mechanical stimuli in vivo, while the silencing of SNAP25 had the opposite effect. These results suggest that SNAP25 plays a crucial role in sensory functional recovery following spinal cord injury (SCI). Our study therefore provides a novel target for the management of SCI for sensory dysfunction.

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Acknowledgments

We wish to thank Ms Kate Rees in School of Pharmacy and Medical Sciences in University of South Australia for critical reading the manuscript. This research was supported by a grant from the China National Science Foundation (No. 81271358, 81070991).

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Anesthesiology and Institute of Neurological Disease, Translation Neuroscience Center, The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Wei Wang, Yang Xu, Ran Liu, Wei Liu, Jin Liu & Ting-Hua Wang

  2. Institute of Neuroscience, Kunming Medical University, Kunming, 650031, People’s Republic of China

    Fang Wang, Jia Liu, Wei Zhao, Qi Zhao & Bao-Jiang Qian

  3. Department of Neurosurgery, Translation Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, People’s Republic of China

    Mu He

  4. Department of Histology and Embryology, Sichuan University, Chengdu, 610041, People’s Republic of China

    Su-Juan Liu

  5. School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA, 5000, Australia

    Xin-Fu Zhou

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  1. Wei Wang
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Correspondence to Ting-Hua Wang.

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Wei Wang and Fang Wang contributed equally to this work.

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Wang, W., Wang, F., Liu, J. et al. SNAP25 Ameliorates Sensory Deficit in Rats with Spinal Cord Transection. Mol Neurobiol 50, 290–304 (2014). https://doi.org/10.1007/s12035-014-8642-8

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  • DOI: https://doi.org/10.1007/s12035-014-8642-8

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