FGF-2 Low Molecular Weight Selectively Promotes Neuritogenesis of Motor Neurons In Vitro
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In this study, we screened in vitro the different capabilities of trophic factors with promising effect for enhancing selective regeneration and thus promoting specific reinnervation of target organs after peripheral nerve regeneration. We found that FGF-2 (18 kDa) was the trophic factor that exerted the most selective effect in promoting neurite outgrowth of spinal motoneurons both in terms of elongation and arborization. The mechanism underlying this effect on neuritogenesis seems related to FGF-2 enhancing the interaction between FGFR-1 and PSA-NCAM. The interaction of these two receptors is important during the early stages of neuritogenesis and pathfinding, while integrin alpha7B subunit seems to play a role during neurite stabilization.
KeywordsAxonal regeneration Motor neuron Sensory neuron In vitro cultures FGF-2 Cell adhesion molecules FGFR-1
This research was supported by grants FP7-MC-214003-2 (Marie Curie Initial Training Network AXREGEN) and FP7-278612 (BIOHYBRID) from the EU, grant PI080598 and TERCEL and CIBERNED funds from the Fondo de Investigación Sanitaria of Spain. The authors thank Dr Rubèn López-Vales for expert support in the co-immunoprecipitation assay and the technical help of Monica Espejo, Jessica Jaramillo and Marta Morell. The anti-alpha7B antibody was a generous gift of Prof. Guido Tarone, Universitá di Torino. The RT97 antibody was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biology.
- 10.Cassens C, Kleene R, Xiao MF, Friedrich C, Dityateva G, Schafer-Nielsen C, Schachner M (2010) Binding of the receptor tyrosine kinase TrkB to the neural cell adhesion molecule (NCAM) regulates phosphorylation of NCAM and NCAM-dependent neurite outgrowth. J Biol Chem 285(37):28959–28967PubMedCrossRefGoogle Scholar
- 29.Velling T, Collo G, Sorokin L, Durbeej M, Zhang H, Gullberg D (1996) Distinct alpha 7A beta 1 and alpha 7B beta 1 integrin expression patterns during mouse development: alpha 7A is restricted to skeletal muscle but alpha 7B is expressed in striated muscle, vasculature, and nervous system. Dev Dyn 207(4):355–371PubMedCrossRefGoogle Scholar
- 31.Song WK, Wang W, Sato H, Bielser DA, Kaufman SJ (1993) Expression of alpha 7 integrin cytoplasmic domains during skeletal muscle development: alternate forms, comformational change, and homologies with serine/threonine kinases and tyrosine phosphatases. J Cell Sci 106(Pt4):1139–1152PubMedGoogle Scholar