Advertisement

In vitro bioactivity evaluation of \(\upalpha \)-calcium sulphate hemihydrate and bioactive glass composites for their potential use in bone regeneration

  • Yanyan Zheng
  • Chengdong Xiong
  • Dujuan Zhang
  • Lifang Zhang
Article
  • 38 Downloads

Abstract

To combine the self-setting property of \(\upalpha \)-calcium sulphate hemihydrate (\(\upalpha \)-CSH) with the bioactive property of bioactive glass (BG), BG was added into \(\upalpha \)-CSH to prepare \(\upalpha \)-CSH/BG composites. The in vitro bioactivity and cytocompatibility of the \(\upalpha \)-CSH/BG composites were assessed by soaking the composites in simulated body fluid (SBF) and co-culturing with the osteoblasts, respectively. Formation of a bone-like apatite layer on the composite surface was studied by X-ray diffraction (XRD), Fourier transform infrared spectrometry (FTIR), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). Variations in ionic concentration and pH of the SBF solution were detected. The incorporation of BG into \(\upalpha \)-CSH, effectively compensated for the pH decrease caused by the dissolution of \(\upalpha \)-CSH and the ion exchange. Osteoblast-like cells (MG63) were cultured on the samples, and the MTT results confirmed that the composites containing BG were more favourable for the proliferation of these cells. Hence, \(\upalpha \)-CSH/BG composites might have great potential for the use as a bone regeneration material.

Keywords

Bioactive glass \(\upalpha \)-calcium sulphate hemihydrate composite bioactivity cytocompatibility 

Notes

Acknowledgements

This work was supported financially by the Doctoral Program of North Sichuan Medical College (No. CBY16-QD-02), the North Sichuan Medical College Scientific Research and Development Projects (No. CBY16-A-ZD02), and the key project of ‘Western Light’ Foundation of Chinese Academy of Sciences.

References

  1. 1.
    Huan Z and Chang J 2007 Acta Biomater. 3 952CrossRefGoogle Scholar
  2. 2.
    Keya M, Feihu Z, Fuzai C, Jiangtao L, Xijun H, Peng L et al 2013 Bio-Med. Mater. Eng. 23 197Google Scholar
  3. 3.
    Pecora G, Andreana S, Margarone III J E, Covani U and Sottosanti J S 1997 Or. Surg. Or. Med. Or. Pathol. 84 424CrossRefGoogle Scholar
  4. 4.
    Chen J, Gao J, Yin H, Liu F, Wang A, Zhu Y et al 2013 Mater. Sci. Eng. C 33 3256CrossRefGoogle Scholar
  5. 5.
    Hughes E, Yanni T, Jamshidi P and Grover L M 2015 Adv. Appl. Ceram. 114 65CrossRefGoogle Scholar
  6. 6.
    Thomas M V and Puleo D A 2009 J. Biomed. Mater. Res. Part B 88 597CrossRefGoogle Scholar
  7. 7.
    Stubbs D, Deakin M, Chapman-Sheath P, Bruce W, Debes J, Gillies R et al 2004 Biomaterials 25 5037CrossRefGoogle Scholar
  8. 8.
    Orsini G, Ricci J, Scarano A, Pecora G, Petrone G, Iezzi G et al 2004 J. Biomed. Mater. Res. Part B 68B 199CrossRefGoogle Scholar
  9. 9.
    Cabanas M V, Rodriguez-Lorenzo L M and Vallet-Regi M 2002 Chem. Mater. 14 3550CrossRefGoogle Scholar
  10. 10.
    Hench L L, Splinter R J, Allen W and Greenlee T 1971 J. Biomed. Mater. Res. 5 117CrossRefGoogle Scholar
  11. 11.
    Zhang D J, Zhang L F, Xiong Z C, Bai W and Xiong C D 2009 J. Mater. Sci. Mater. Med. 20 1971CrossRefGoogle Scholar
  12. 12.
    Bellucci D, Sola A and Cannillo V 2014 Biomed. Mater. 9 015005CrossRefGoogle Scholar
  13. 13.
    Jones J R2013 Acta Biomater. 9 4457CrossRefGoogle Scholar
  14. 14.
    Kokubo T and Yamaguchi S 2016 Acta Biomater. 44 16CrossRefGoogle Scholar
  15. 15.
    Kaur G, Pandey O P, Singh K, Homa D, Scott B and Pickrell G 2014 J. Biomed. Mater. Res. Part A 102 254CrossRefGoogle Scholar
  16. 16.
    Snyders Jr R V, Eppley B L, Krukowski M and Delfino J J 1993 J. Oral Maxil. Surg. 51 517CrossRefGoogle Scholar
  17. 17.
    Ramila A, Balas F and Vallet-Regi M 2002 Chem. Mater. 14 542CrossRefGoogle Scholar
  18. 18.
    Kokubo T and Takadama H 2006 Biomaterials 27 2907CrossRefGoogle Scholar
  19. 19.
    Zheng Y, Xiong C, Zhang S, Li X and Zhang L 2015 Mater. Sci. Eng. C 55 512CrossRefGoogle Scholar
  20. 20.
    Zheng Y, Xiong C, Li X and Zhang L 2014 Appl. Surf. Sci. 320 93CrossRefGoogle Scholar
  21. 21.
    Zheng Y, Xiong C and Zhang L 2014 Mater. Lett. 126 147CrossRefGoogle Scholar
  22. 22.
    Shen Y, Liu W, Lin K, Pan H, Darvell B W, Peng S et al 2011 Langmuir 27 2701CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  1. 1.School of Basic Medical SciencesNorth Sichuan Medical CollegeNanchongPeople’s Republic of China
  2. 2.Chengdu Institute of Organic ChemistryChinese Academy of SciencesChengduPeople’s Republic of China

Personalised recommendations