Skip to main content


Log in

Chitosan-encapsulated ZnS : M (M: Fe3+ or Mn2+) quantum dots for fluorescent labelling of sulphate-reducing bacteria

  • Published:
Bulletin of Materials Science Aims and scope Submit manuscript


Chitosan-encapsulated Mn2+ and Fe3+-doped ZnS colloidal quantum dots (QDs) were synthesized using chemical precipitation method. Though there are many reports on bio-imaging applications of ZnS QDs, the present study focussed on the new type of microbial-induced corrosive bacteria known as sulphate-reducing bacteria, Thiobacillus novellus. Sulphate-reducing bacteria can obtain energy by oxidizing organic compounds while reducing sulphates to hydrogen sulphide. This can create a problem in engineering industries. When metals are exposed to sulphate containing water, water and metal interacts and creates a layer of molecular hydrogen on the metal surface. Sulphate-reducing bacteria then oxidize the hydrogen while creating hydrogen sulphide, which contributes to corrosion for instance, in pipelines of oil and gas industries. In this study, detection and labelling of sulphate-reducing bacteria is demonstrated using fluorescent QDs. Chitosan capped ZnS QDs were synthesized using dopants at different doping concentrations. UV–Vis spectroscopy, XRD and FTIR characterizations were done to identify the optical band gap energy, crystal planes and determine the presence of capping agent, respectively. The morphology and the average particle size of 3.5 ± 0.2 nm were analysed using TEM which substantiated UV–Vis and XRD results. Photoluminescence spectroscopy detected the bacteria attachment to the QDs by showing significant blue shift in bacteria conjugated ZnS QDs. Fluorescence microscopy confirmed the fluorescent labelling of QDs to Thiobacillus novellus bacteria cells making them ideal for bio-labelling applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11

Similar content being viewed by others


  1. Schmid Gunter 2004 Nanoparticles—from theory to application (Weinheim: Wiley)

  2. Jaszowiec K B 2001 Acta Phys. Polonica A 100 275

  3. Azpiroz J M, Lopez X, Ugalde J M and Infante I 2012 J. Phys. Chem. C 116 2740

    Article  Google Scholar 

  4. Paul A 2000 Pure Appl. Chem. 72 3

    Google Scholar 

  5. Pan Z, Mora-Seró I, Shen Q, Zhang H, Li Y, Zhao K, Wang J, Zhong X and Bisquert J 2014 J. Am. Chem. Soc. 136 9203

    Article  Google Scholar 

  6. Willner Freeman R and Itamar 2012 Chem. Soc. Rev. 41 4067

    Article  Google Scholar 

  7. Chaniotakis Frasco M F and Nikos 2009 Sensors 9 7266

    Article  Google Scholar 

  8. Sun K, Vasudev M, Jung H -S, Yang J, Kar A, Li Y et al 2009 Microelectron. J. 40 644

    Article  Google Scholar 

  9. Mazumder S, Sarkar J, Dey R, Mitra M K, Mukherjee S and Das G C 2010 J. Exp. Nanosci. 5 438

    Article  Google Scholar 

  10. Paul A 2004 Nat. Biotechnol. 22 47

  11. Xu Z, Li B, Tang W, Chen T, Zhang H and Wang Q 2011 Colloid. Surf. B: Biointerfaces 88 51

  12. Erwin S C, Zu L, Haftel M I, Efros A L, Kennedy T A and Norris D J 2005 Nat. Lett. 436 91

  13. Dixit N, Anasane N, Chavda M, Bodas D and Soni H P 2012 Cryst. Res. Technol. 47 1105

    Article  Google Scholar 

  14. Hu H and Zhang W 2006 Opt. Mater. 28 536

    Article  Google Scholar 

  15. Mazumder S, Dey R, Mitra M K, Mukherjee S and Das G C 2009 J. Nanomater. 2009 815734

  16. ChineduI O 2012 Int. Scholar. Res. Network 570143 10

    Google Scholar 

  17. Dinh H T, Kuever J, Mußmann M, Hassel A W, Stratmann M and Widdel F 2004 Nature 427 829

    Article  Google Scholar 

  18. Ramanery F P, Mansur A A P and Mansur H S 2013 Nanoscale Res. Lett. 512 8

    Google Scholar 

  19. Rajabi H R, Khani O, Shamsipur M and Vatanpour V 2013 J. Hazard. Mater. 250 370

    Article  Google Scholar 

  20. Mansur H S, Mansur A A P, Araújo A S and Lobato Z I P 2015 Green Chem. 17 1820

    Article  Google Scholar 

  21. Ramanery F P, Mansur A A P, Borsagli F G L M and Mansur H S 2014 J. Nanopart. Res. 2504 1

    Article  Google Scholar 

  22. Tan W B, Huang N and Zhang Y 2007 J. Colloid Interf. Sci. 310 464

    Article  Google Scholar 

  23. Masanori T 2002 J. Lumin. 100 163

  24. Joicy S, Saravanan R, Prabhu D, Ponpandiand N and Thangadurai P 2014 Royal Soc. Chem. Adv. 4 44592

    Google Scholar 

  25. Khani O, Rajabi H R, Yousefi M H, Khosravi A A, Jannesari M and Shamsipur M 2011 Spectrochim. Acta Part A 79 361

  26. Slack G A, Ham F S and Chrenko R M 1966 Phys. Rev. 152 376

  27. Bhadraa P, Mitrac M K, Dasa G C, Deya R and Mukherjeeb S 2011 Mater. Sci. Eng. C 31 929

    Article  Google Scholar 

  28. Hahn M A, Tabb J S and Krauss T D 2005 Anal. Chem. 77 4861

  29. Kloepfer J A, Mielke R E, Wong M S, Nealson K H, Stucky G and Nadeau J L 2003 Appl. Environ. Microbiol. 69 4205

    Article  Google Scholar 

  30. Kloepfer J A, Mielke R E and Nadeau J L 2005 Appl. Environ. Microbiol. 71 2548

    Article  Google Scholar 

Download references


We acknowledge the Department of Chemical Engineering, Department of Physics, Department of Biology and Department of Pharmacy at BITS Pilani, Rajasthan, Department of Metallurgical and Materials Science, School of Material Science and Nanotechnology at Jadavpur University, Kolkata, and Materials Research centre, MNIT, Jaipur, for availing their research facility.

Author information

Authors and Affiliations


Corresponding author

Correspondence to SONAL MAZUMDER.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

RAGHURAM, H.S., PRADEEP, S., DASH, S. et al. Chitosan-encapsulated ZnS : M (M: Fe3+ or Mn2+) quantum dots for fluorescent labelling of sulphate-reducing bacteria. Bull Mater Sci 39, 405–413 (2016).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: