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Hydrophobic nano-carrier for lysozyme adsorption

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Abstract

In this work, poly(HEMA–APH) nanoparticles were synthesized by surfactant-free emulsion polymerization technique. Magnetic behaviour was introduced by simple addition of Fe3O4 into the polymerization medium. Characterization of the nanoparticle was carried out by FTIR, ESR, SEM, AFM and EDX analyses. These synthesized magnetic nanoparticles were used for adsorption of lysozyme. For this purpose, adsorption conditions were optimized and maximum lysozyme binding capacity was found to be 278.8 mg g−1 polymer in pH 7.0 phosphate buffer at 25∘C. Desorption and reusability properties of the nanoparticles were investigated and lysozyme adsorption efficiency did not change significantly at the end of the 10 successive reuses.

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References

  1. Jing T, Du H, Dai Q, Xia H, Niu J, Hao Q, Mei S and Zhou Y 2010 Biosens. Bioelectron. 26 301

    Article  Google Scholar 

  2. Freitas D S and Abrahano-Neto J 2010 Int. J. Pharm. 392 111

    Article  Google Scholar 

  3. Zhang G, Cao Q, Li N, Li K and Liu F 2011 Talanta 83 1515

    Article  Google Scholar 

  4. Murakami F, Sasaki T and Sugahara T 1997 Cytotechnology 24 177

  5. Uygun D A, Karagözler A A, Akgöl S and Denizli A 2009 Mat. Sci. Eng. C 29 2165

    Article  Google Scholar 

  6. Cömert Ş C and Odabaşı M 2014 Mat. Sci. Eng. C 34 1

    Article  Google Scholar 

  7. Altıntaş E B and Denizli A 2006 Int. J. Biol. Macromol. 38 99

    Article  Google Scholar 

  8. Odabaşı M and Denizli A 2004 J. Appl. Polym. Sci. 93 719

    Article  Google Scholar 

  9. Odabaşı M, Say R and Denizli A 2007 Mat. Sci. Eng. C 27 90

    Article  Google Scholar 

  10. Hearn M T W 2002 in HPLC of biological macromolecules (eds) K M Gooding and F E Regnier (New York: Marcel Dekker) Chapter 5

  11. Jungbauer A and Feng W 2002 in: HPLC of biological macromolecules (eds) K M Gooding and F E Regnier (New York: Marcel Dekker)

  12. Queiroz J A, Tomaz C T and Cabral J M S 2005 J. Biotechnol. 87 143

    Article  Google Scholar 

  13. Jungbauer A, Machold C and Hahn R 2005 J. Chromatogr. A 1079 221

    Article  Google Scholar 

  14. Shepard C C and Tiselius A 1949 Discussions of the Faraday Society, Vol. 7 (London: Hazell Watson and Winey) p 275

  15. Er-el Z and Shaltiel S 1974 FEBS Lett. 40 142

    Article  Google Scholar 

  16. Shaltiel S and Er-el Z 1973 Proc. Natl. Acad. Sci. 70 778

    Article  Google Scholar 

  17. Hofstee B H J 1973 Anal. Biochem. 52 430

    Article  Google Scholar 

  18. Hjerten S, Rosengren J and Pahlman S J 1974 J. Chromatogr. A 101 281

    Article  Google Scholar 

  19. Mahn A, Lienqueo M E and Salgado J C 2009 J. Chromatogr. A 1216 1838

    Article  Google Scholar 

  20. Queiroz J A, Tomaz C T and Cabral J M S 2001 J. Biotechnol. 87 143

    Article  Google Scholar 

  21. Van Oss C J, Good R J and Chaudhury M K 1986 J. Colloid Interface Sci. 111 378

    Article  Google Scholar 

  22. Melander W R, Conrradi D and Horvath C 1984 J. Chromatogr. 317 67

    Article  Google Scholar 

  23. Diogo M M, Queiroz J A and Prazeres D M F 2002 J. Chromatogr. A 944 119

    Article  Google Scholar 

  24. Arakawa T and Timasheff S N 1982 Biochemistry 21 6545

    Article  Google Scholar 

  25. Freitas S S, Santos J A L and Prazeres D M F 2009 Sep. Purif. Technol. 65 95

    Article  Google Scholar 

  26. Huang S -H and Juang R -S 2011, J. Nanopart. Res. 13 4411

    Article  Google Scholar 

  27. Namdeo M and Bajpai S K 2009 J. Mol. Catal. B 59 134

    Article  Google Scholar 

  28. Sarı M, Akgöl S, Karataş M and Denizli A 2006 Ind. Eng. Chem. Res. 45 3036

    Article  Google Scholar 

  29. Akgöl S, Bereli N and Denizli A 2005 Macromol. Biosci. 5 786

    Article  Google Scholar 

  30. Akgöl S, Türkmen D and Denizli A 2004 J. Appl. Polym. Sci. 93 2669

    Article  Google Scholar 

  31. Halling P J and Dunnill P 1980 Enzyme Microb. Technol. 2 2

    Article  Google Scholar 

  32. Cao L 2005 Curr. Opin. Biotechnol. 9 217

    Article  Google Scholar 

  33. Sheldon R A 2007 Adv. Synth. Catal. 49 1289

    Article  Google Scholar 

  34. Jesionowski T, Zdarta J and Krajewska B 2014 Adsorption 20 801

    Article  Google Scholar 

  35. Hubacher M H 1951 J. Am. Chem. Soc. 73 5885

    Article  Google Scholar 

  36. Avcıbaşı U, Avcıbaşı N, Akalın H A, Ediz M, Demiroğlu H, Gümüşer F G et al 2013, J. Nanopart. Res. 15 2021

    Article  Google Scholar 

  37. Uygun M, Uygun D A, Altunbaş C, Akgöl S and Denizli A 2014 Sep. Sci. Technol. 49 1270

    Article  Google Scholar 

  38. Shugar D 1952 Biochim. Biophys. Acta 8 302

    Article  Google Scholar 

  39. Xie W and Ma N 2009 Energy Fuel 23 1347

    Article  Google Scholar 

  40. Liu T, Zhao Y, Wang X, Li X and Yan Y 2013 Bioresour. Technol. 132 99

    Article  Google Scholar 

  41. Cui R, Bai C, Jiang Y, Hu M, Li S and Shai Q 2015 Chem. Eng. J. 259 640

    Article  Google Scholar 

  42. Yılmaz F, Bereli N, Yavuz H and Denizli A 2009 Biochem. Eng. J. 43 272

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Ege University, 35040, İzmir, Turkey

    CANAN ALTUNBAŞ, FULDEN ZEYNEP URAL & SİNAN AKGÖL

  2. Kocarlı Vocational and Training School, Adnan Menderes University, 09010, Aydın, Turkey

    MURAT UYGUN

  3. Ege Higher Vocational School, Ege University, 35040, İzmir, Turkey

    NESİBE AVCIBAŞI

  4. Department of Chemistry, Faculty of Arts and Science, Celal Bayar University, 45140, Manisa, Turkey

    UĞUR AVCIBAŞI

  5. Department of Chemistry, Faculty of Arts and Science, Adnan Menderes University, 09010, Aydın, Turkey

    DENİZ AKTAŞ UYGUN

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  1. CANAN ALTUNBAŞ
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Correspondence to DENİZ AKTAŞ UYGUN.

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ALTUNBAŞ, C., URAL, F.Z., UYGUN, M. et al. Hydrophobic nano-carrier for lysozyme adsorption. Bull Mater Sci 39, 353–359 (2016). https://doi.org/10.1007/s12034-016-1177-z

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  • DOI: https://doi.org/10.1007/s12034-016-1177-z

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