Abstract
The aim of the present work was to study the in vitro corrosion resistance in Hank’s solution and biocompatibility of indigenized low-cost Ni-free nitrogen containing austenitic stainless steels (HNSs) and to compare with conventionally used 316L and 316LVM. The electrochemical behaviour was assessed using electrochemical impedance spectroscopy, potentiostatic polarization and scanning electron microscopy. The MTT assay [3-(4,5-dimethythiazol 2-yl)-2,5-diphenyltetrazolium bromide] was performed using Dalton’s lymphoma cell line for cytotoxicity evaluation and cell adhesion test. The resistance of surface film was raised by increasing nitrogen content in stainless steel (SS). The corrosion current density was decreased with increase in nitrogen content and corrosion potentials for HNS were observed to be more positive. Shallower and smaller pits were associated with HNS, indicating that nitrogen suppresses the pit formation. The HNS had higher cell proliferation and cell growth and it increases by increasing the nitrogen content. The surface wettability of the alloys was also investigated by water contact-angle measurements. The value of contact angles was found to decrease with increase in nitrogen content. This indicates that the hydrophilic character increases with increasing nitrogen content, which is further attributed to enhance the surface free energy that would be conducive to cell adhesion, which in turn increases the cell proliferation.
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ASTM F 2581-07, Standard specification for wrought nitrogen strengthened 11 manganese-17 chromium-3 molybdenum low-nickel stainless steel alloy bar and wire for surgical implants (UNS S29225)
Azumi K, Ohtsuka T and Sato N 1986 Trans. Jpn. Inst. Met. 27 382
Abreu C M, Cristobal M J, Losada R, Novoa X R, Pena G and Perez M C 2004 J. Electroanal. Chem. 572 335
Baier R E, Meyer A E, Natiella J R, Natiella R R and Carter J M 1984 J. Biomed. Mater. Res. 18 327
Bal W, Kozoowski H and Kasprzak K 2000 J. Inorg. Biochem. 79 213
Baba H, Kodama T and Katada Y 2002 Corros. Sci. 44 2393
Buhagiar J, Bell T, Sammons R and Dong H 2011 J. Mater. Sci: Mater. Med. 22 1269
Clayton C R 1986 Passivity mechanisms in stainless steels: Mo-N synergism, Report No. N00014-85-K-0437. New York
Disegi J A and Eschbach L 2000 Injury 31 4
Fini M, Aldini N, Torricelli P, Glavaresi G, Borsari V, Lenger H, Bernauer J, Glardino R, Chiesa R and Cigada A 2003 Biomaterials 24 4929
Fini M et al 2004 J. Biomed. Mater. Res. B Appl. Biomater. B71 30
Gurappa I and Kumar M K 2000 Evaluation of corrosion resistance of plasma sprayed alumina, magnesia-stabilized zirconia and yttrium-stabilized zirconia coatings on 316 stainless steel for biomedical applications. Proceedings of Global 2000 Corrosion Meeting (Mumbai, India: Quest Publications), vol 1, p 561
Ge H, Zhou G and Wu W 2001 Appl. Surf. Sci. 211 321
Gurappa I 2002 Mater. Charac. 49 73
Ha H Y, Jang H, Kwon H S and Kim S J 2009 Corros. Sci. 51 48
IARC 1999 Monographs on the evaluation of carcinogenic risks to humans: surgical implants and other foreign bodies (Lyon) vol. 74, p 65
Jorcin J B, Orazem M E, Pebere N and Tribollet B 2006 Electrochim. Acta 51 1473
Kuroda D, Hiromoto S, Hanawa T and Katada Y 2002 Mater. Trans. 43 3100
Kennedy S B, Washburn N R, Simon Jr C G and Amis E J 2006 Biomaterials 27 3817
Lu Y C, Bandy R, Clayton C R and Newman R C 1983 J. Electrochem. Soc. 130 1774
Lee J B 2006 Mater. Chem. Phys. 99 224
Mudali U K, Reynders B and Stratmann M 1994 Mat. Sci. Forum. 723 185
Menzel J, Kirschner W and Stein G 1996 ISIJ Int. 36 893
Mudali U K, Dayal R K, Gnanamoorthy J B and Rodriguez P 1996 Mat. Trans. JIM 37 1568
Milosev I and Strehblow H H 2000 J. Biomed. Mater. Res. 52 404
Ma T, Wan P, Cui Y, Zhang G, Li J, Liu J, Ren Y, Yang K and Lu L 2012 J. Mater. Sci. Technol. 28 647
Newman R C, Lu Y C, Bandy R and Clayton C R 1984 Proceedings of the ninth International congress on metallic corrosion (Ottawa, Canada: National Research Council), vol 4, p 394
Newman R C and Shahrabi T 1987 Corros. Sci. 27 827
Osozawa K, Okato N, Fukase Y, Yokota K and Boshoku-Gijyutsu 1975 Corros. Eng. 24 1
Osozawa K, Okato N, Stahele R W and Okada H (eds) 1976 in Passivity and its breakdown on iron and iron base alloys (NACE), p 135
Olsson C O A 1995 Corros. Sci. 37 467
Ohno H, Tanabe H, Sakai A, Misawa T and Zairyo-to-Kankyo 1998 Corros. Eng. 47 584
Omanovic S and Roscoe S G 1999 Langmuir 15 8315
Omanovic S and Roscoe S G 2000 J. Coll. Interf. Sci. 227 452
Olsson C O A and Landolt D 2003 Electrochimica Acta 48 1093
Palit G C, Kain V and Gadiyar H S 1993 Corrosion 49 977
Pattersson R F A J 1999 Corros. Sci. 41 1639
Ren Y, Wan P, Liu F, Zhang B and Yang K 2011 J. Mater. Sci. Technol. 27 325
Sumita M 1997 Orthop. Surg. 48 927
Schmidt C, Ignatius A and Claes L E 2001 J. Biomed. Mater. Res. 54 209
Shih C C, Shih C M, Su Y Y and Lin S J 2003 J. Biomed. Mater. Res.: Part A 67 1320
Shahryari A, Omanovic S and Szpunar J A 2008 Mater. Sci. Eng. C28 94
Sudesh T L, Wijesinghe L and Blackwood D J 2008 Corros. Sci. 50 23
Tschiptschin A, Aidar C, Neto F and Alonso-Falleiros N 1999 Mater. Sci. Forum 569 318
Thomann U I and Uggowitzer P J 2000 Wear 239 48
Takazawa K, Miyagawa H and Hariya A 2003 Jpn. Soc. Artif. Organs. 6 71
Talha M, Behera C K and Sinha O P 2012 J. Chem. Pharmaceut. Res. 4 203
Vanini A S, Audouard J P and Marcus P 1994 Corros. Sci. 36 1825
Vahter M, Berglund M, Akesson A and Liden C 2002 Environ. Res. 88 145
Woodman J L, Jacobs J J, Galante, J O and Urban R M 1984 J. Orthop. Res. 1 421
Wan P, Ren Y, Zhang B and Yang K 2010 Mater. Sci. Eng. C30 1183
Yu Z S 1987 Applications of rare earths in iron and steel (in Chinese), (Beijing: Metallurgical Industry Press) p 282
Yamamoto A, Kohyama Y, Kuroda D and Hanawa T 2004 Mater. Sci. Eng. C24 737
Zhao G, Raines A L, Wieland M, Schwartz Z and Boyan B D 2007 Biomaterials 28 2821
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Talha, M., Behera, C.K. & Sinha, O.P. Promising in vitro performances of nickel-free nitrogen containing stainless steels for orthopaedic applications. Bull Mater Sci 37, 1321–1330 (2014). https://doi.org/10.1007/s12034-014-0078-2
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DOI: https://doi.org/10.1007/s12034-014-0078-2