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Isolation and Characterization of an Antioxidant Aryl Polyene Pigment from Antarctic Bacterium Lysobacter sp. A03

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Abstract

Lysobacter is known as a bacterial genus with biotechnological potential, producing an array of enzymes, antimicrobial metabolites, and bioactive antioxidant compounds, including aryl polyene (APE) pigments that have been described as protecting substances against photooxidative damage and lipid peroxidation. In this study, the pigment extracted from keratinolytic Lysobacter sp. A03 isolated from Antarctic environment was characterized. The results of KOH test, UV–vis spectroscopy, CIELAB color system, 1H-NMR, and FTIR-ATR spectroscopy suggest the pigment is a yellow xanthomonadin-like pigment. The in vitro antioxidant activity of the pigment was confirmed by the scavenging of ABTS and DPPH radicals. In silico analysis of the genome through antiSMASH software was also performed and the secondary metabolite gene clusters for APE and resorcinol synthesis were identified, suggesting that proteins responsible for the pigment biosynthesis are encoded in Lysobacter A03 genome.

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Data Availabitily

The genome data from Lysobacter sp. strain A03 is publicy available at GenBank (https://www.ncbi.nlm.nih.gov/genbank/), accession number JXSS00000000.

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Acknowledgements

This work was supported by CNPq (grant 308880/2021-8) and CAPES (Brasilia, Brazil). MEPJ was a former recipient of a PhD fellowship from COLFUTURO (Bogotá, Colombia).

Funding

Funding was provided by Conselho Nacional de Desenvolvimento Científico e Tecnológico (Grant No: 308880/2021-8) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

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Authors and Affiliations

  1. Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, ICTA-UFRGS, Av. Bento Gonçalves 9500, Porto Alegre, 91501-970, Brazil

    Maria Elisa Pailliè-Jiménez, Jamile Queiroz Pereira, Eliseu Rodrigues & Adriano Brandelli

  2. CMFI Cluster of Excellence, Interfaculty Institute of Microbiology and Medicine, University of Tübingen, 72076, Tübingen, Germany

    Paolo Stincone

  3. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires & Centro de Investigaciones en Hidratos de Carbono (CIHIDECAR, UBA-CONICET), 1428, Buenos Aires, Argentina

    Patricio Román Santagapita

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  1. Maria Elisa Pailliè-Jiménez
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Pailliè-Jiménez, M.E., Stincone, P., Pereira, J.Q. et al. Isolation and Characterization of an Antioxidant Aryl Polyene Pigment from Antarctic Bacterium Lysobacter sp. A03. Mol Biotechnol (2024). https://doi.org/10.1007/s12033-024-01132-7

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