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Application of Computational Techniques in Antibody Fc-Fused Molecule Design for Therapeutics

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Abstract

Since the advent of hybridoma technology in the year 1975, it took a decade to witness the first approved monoclonal antibody Orthoclone OKT39 (muromonab-CD3) in the year 1986. Since then, continuous strides have been made to engineer antibodies for specific desired effects. The engineering efforts were not confined to only the variable domains of the antibody but also included the fragment crystallizable (Fc) region that influences the immune response and serum half-life. Engineering of the Fc fragment would have a profound effect on the therapeutic dose, antibody-dependent cell-mediated cytotoxicity as well as antibody-dependent cellular phagocytosis. The integration of computational techniques into antibody engineering designs has allowed for the generation of testable hypotheses and guided the rational antibody design framework prior to further experimental evaluations. In this article, we discuss the recent works in the Fc-fused molecule design that involves computational techniques. We also summarize the usefulness of in silico techniques to aid Fc-fused molecule design and analysis for the therapeutics application.

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Funding

This work is supported by FRGS (203/CIPPM/6711680; FRGS/1/2018/STG05/USM/02/1) from the Malaysia Ministry of Education.

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  1. Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Minden, Penang, Malaysia

    Chong Lee Ng, Theam Soon Lim & Yee Siew Choong

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  1. Chong Lee Ng
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  2. Theam Soon Lim
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  3. Yee Siew Choong
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CLN drafted the manuscript. TSL and YSC designed and edit the manuscript.

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Correspondence to Yee Siew Choong.

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Ng, C.L., Lim, T.S. & Choong, Y.S. Application of Computational Techniques in Antibody Fc-Fused Molecule Design for Therapeutics. Mol Biotechnol 66, 568–581 (2024). https://doi.org/10.1007/s12033-023-00885-x

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