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Recent Advances in Overexpression of Functional Recombinant Lipases

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Abstract

Heterologous functional expression of the recombinant lipases is typically a bottleneck due to the expression in the insoluble fraction as inclusion bodies (IBs) which are in inactive form. Due to the importance of lipases in various industrial applications, many investigations have been conducted to discover suitable approaches to obtain functional lipase or increase the expressed yield in the soluble fraction. The utilization of the appropriate prokaryotic and eukaryotic expression systems, along with the suitable vectors, promoters, and tags, has been recognized as a practical approach. One of the most powerful strategies to produce bioactive lipases is using the molecular chaperones co-expressed along with the target protein’s genes into the expression host to produce the lipase in soluble fraction as a bioactive form. The refolding of expressed lipase from IBs (inactive) is another practical strategy which is usually carried out through chemical and physical methods. Based on recent investigations, the current review simultaneously highlights strategies to express the bioactive lipases and recover the bioactive lipases from the IBs in insoluble form.

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Funding

This work has received funding from Ministry of Higher Education (MOHE) under Fundamental Research Grant Scheme (FRGS/1/2019/STG05/UPM/02/15).

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Authors and Affiliations

  1. Enzyme and Microbial Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Fatin Liyana Alias, Nima Ghahremani Nezhad, Yahaya M. Normi, Mohd Shukuri Mohamad Ali & Thean Chor Leow

  2. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Fatin Liyana Alias, Nima Ghahremani Nezhad, Yahaya M. Normi & Thean Chor Leow

  3. Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Mohd Shukuri Mohamad Ali

  4. Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia

    Cahyo Budiman

  5. Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Thean Chor Leow

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  1. Fatin Liyana Alias
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FLA, NGN and TCL conceived, designed and wrote the manuscript. YMN, MSMA and CB improved the quality of manuscript by compiling information in the form of tables and figures. All authors read and approved the manuscript.

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Alias, F.L., Nezhad, N.G., Normi, Y.M. et al. Recent Advances in Overexpression of Functional Recombinant Lipases. Mol Biotechnol 65, 1737–1749 (2023). https://doi.org/10.1007/s12033-023-00725-y

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