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Carotenoid Differences and Genetic Diversity in Populations of Sargassum hemiphyllum and Sargassum fusiforme

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Abstract

Sargassum hemiphyllum and Sargassum fusiforme are important benthic seaweeds that grow along the southeastern coast of China. The content of carotenoids in each population was detected by ultra-high performance liquid chromatography (UHPLC). The research results will enrich the theoretical basis and data support concerning the influencing factors of carotenoids in Sargassum. The inter-simple sequence repeat (ISSR) technique was used to study the genetic diversity of four S. hemiphyllum and two S. fusiforme populations, and the results provide a reference for the artificial cultivation of Sargassum. The total carotenoid content of Sargassum ranged from 161.79 ± 4.22 to 269.47 ± 6.15 μg/g. Among the carotenoids, β-carotene and fucoxanthin accounted for 80%, and levels in S. hemiphyllum were generally higher than those in S. fusiforme. The carotenoid contents of S. hemiphyllum from different areas were significantly different (P < 0.05), and the total carotenoids content decreased toward the southern region. The average heterozygosity H ranged from 0.29 to 0.49, and the Shannon diversity index I ranged from 0.44 to 0.69. The polymorphic loci, genetic diversity, and other indicators of S. hemiphyllum populations were higher than those of S. fusiforme, and the diversity of cultivated populations was not significantly lower. The results showed that the genetic variation of Sargassum is limited, and thus, more sexual reproduction can be attempted in breeding. Considering morphological indicators, genetic diversity indexes, and carotenoid content, S. hemiphyllum appears to have a higher commercial development value.

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Acknowledgements

The author is grateful to all authors for their support while conducting this research. They would like to thank Shuang Li for pigment determination. They thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Funding

This project was funded by the Zhejiang Province Nature Science Foundation of China [Grant Number LY22C190002]; National Natural Science Foundation of China [Grant Number 32273155]; Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural (Aquaculture) Varieties (2021C02069-9); Major Scientific and Technological Project of Ningbo (2021Z004, 2021Z103); Scientific and Technological Project of Ningbo (2021S063); China Agriculture Research System of MOF; and MARA, K.C. Wong Magna Fund at Ningbo University.

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Authors and Affiliations

  1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo, 315211, Zhejiang, China

    Rui Xian, Rui Yang, Juanjuan Chen & Haimin Chen

  2. Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, 315211, Zhejiang, China

    Rui Xian, Rui Yang, Qijun Luo, Juanjuan Chen & Haimin Chen

  3. Zhejiang Institute of Marine Aquaculture, Wenzhou, 325005, China

    Peng Zhang

  4. Guangdong Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, Guangdong, China

    Weizhou Chen

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  1. Rui Xian
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Contributions

This study was conducted in cooperation between all authors. RX contributed toward implementation of all experiments and paper writing. RY contributed toward experimental design and paper writing. PZ contributed toward sample collection and algal culture. WC contributed toward sample collection and diversity analysis. QL contributed toward sample collection and germplasm identification. JC contributed toward carotenoid analysis. HC contributed toward carotenoid analysis and diversity analysis.

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Correspondence to Rui Yang.

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Xian, R., Yang, R., Zhang, P. et al. Carotenoid Differences and Genetic Diversity in Populations of Sargassum hemiphyllum and Sargassum fusiforme. Mol Biotechnol 65, 1378–1386 (2023). https://doi.org/10.1007/s12033-022-00644-4

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