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Evaluation of the Expression of miRNAs, LncRNAs, and their Target Gene, Caspase 3 in Glioblastoma Multiform: A Case–Control Study

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Abstract

Glioblastoma multiform (GBM) is an invasive cancer that causes high mortality in patients. Disruption of the apoptosis process is one of the main pathogenesis of the disease. Recently, LncRNAs and miRNAs have been shown to play an important role in the process of apoptosis. To follow the aim of study, 100 patients participated in the two groups of 50 individuals, including 50 GBM patients and 50 healthy individuals as the control group. Mononuclear cells were isolated from peripheral blood samples and RNA extraction was done. The expression changes of miR-17-5p, miR-20-5p, LINC01605, FAS-AS1, and Caspase 3 were examined using RT-PCR in both groups. Expression of LINC01605, miR-20-5p, and miR-17-5p increased in patients, while Caspase 3 and FAS-AS1 decreased; the difference was statistically significant between the two groups. In addition, it was found that these factors have the appropriate sensitivity and specificity as diagnostic markers. Finally, It is suggested to use the LINC01605, FAS-AS1, miR-20-5p, miR-17-5p, and Caspase 3 as apoptosis predictors in the GM patients.

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Data Availability

The datasets used analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

GBM:

Glioblastoma multiform

Ct:

Cycle threshold

AUC:

Area under the curve

ROC:

Receiver operating characteristic

sFas:

Soluble Fas receptor

RRM2:

Ribonucleotide reductase subunit M2

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Acknowledgements

We wish to thank our colleagues in Tehran University.

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Authors and Affiliations

  1. Department of Cellular and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran

    Shirin Setoodeh Haghighi & Nasrin Motamed

  2. Skull Base Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Sayyed Mohammad Hossein Ghaderian

  3. Department of Pathology, School of Medicine, Shohada-E-Tajrish Educational Hospital, ShahidBeheshti University of Medical Sciences, Tehran, Iran

    Azadeh Rakhshan

  4. Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, 14155-6455, Iran

    Nasrin Motamed

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  1. Shirin Setoodeh Haghighi
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Correspondence to Nasrin Motamed.

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Haghighi, S.S., Ghaderian, S.M.H., Rakhshan, A. et al. Evaluation of the Expression of miRNAs, LncRNAs, and their Target Gene, Caspase 3 in Glioblastoma Multiform: A Case–Control Study. Mol Biotechnol 65, 1444–1452 (2023). https://doi.org/10.1007/s12033-022-00632-8

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  • DOI: https://doi.org/10.1007/s12033-022-00632-8

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