Abstract
Growing evidence has suggested that lncRNAs play a significant role in the development of colorectal adenocarcinoma. LncRNA LINC02535 was a potential novel lncRNA marker of neoplastic processes of the colon. Nevertheless, the function and mechanisms of LINC02535 in colorectal adenocarcinoma remain unclear. Proteins levels were measured by western blotting. EdU, CCK-8, Transwell, and wound healing assays were performed to investigate the function of LINC02535 in colorectal adenocarcinoma. The distribution of LINC02535 in cells was evaluated by subcellular fractionation assay. The interaction among RNAs was identified by luciferase reporter and RIP assays. In this study, our findings revealed that LINC02535 was highly expressed in colorectal adenocarcinoma cells. Knockdown of LINC02535 inhibited colorectal adenocarcinoma cell proliferation, migration, and invasion. Mechanistically, LINC02535 bound with miR-30d-5p and worked as a competing endogenous RNA to facilitate the expression of messenger RNA chromodomain helicase DNA-binding protein 1 (CHD1). miR-30d-5p directly targeted the sequence of CHD1 3′-untranslated region. Notably, CHD1 upregulation abolished the suppressive influence of LINC02535 inhibition on the malignant phenotypes of colorectal adenocarcinoma cells. Overall, it was disclosed that LINC02535 played an oncogenic role in colorectal adenocarcinoma progression by sponging miR-30d-5p to upregulate CHD1 expression.
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The datasets used during the current study are available from the corresponding author on reasonable request.
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12033_2022_628_MOESM1_ESM.tif
Supplementary file1 (TIF 175 KB) Effect of LINC02535 on NCM460 cell proliferation. A. qRT-PCR analysis for assessing transfection efficiency of LINC02535 knockdown in NMC460 cells. B. CCK-8 assay for assessing the viability of NCM460 cells. C-D. EdU assay for evaluating proliferation of NCM460 cells
12033_2022_628_MOESM2_ESM.tif
Supplementary file2 (TIF 179 KB) Effect of CHD1 on NCM460 cell proliferation. A. qRT-PCR analysis for assessing transfection efficiency of CHD1 overexpression in NMC460 cells. B. CCK-8 assay for examining the viability of NCM460 cells. C-D. EdU assay for evaluating proliferation of NCM460 cells
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Li, J., Xu, J., Zheng, S. et al. LncRNA LINC02535 Induces Colorectal Adenocarcinoma Progression via Modulating miR-30d-5p/CHD1. Mol Biotechnol 65, 1346–1358 (2023). https://doi.org/10.1007/s12033-022-00628-4
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DOI: https://doi.org/10.1007/s12033-022-00628-4