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Fabrication of a Patterned Scaffold Using Soft Lithography Technique to be Used in Cell Growth Applications

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Abstract

In recent years, within tissue engineering, cell growth on patterned surfaces have gained significant attention. Growing cells in patterns is important to manufacture polymeric tissues that can be used within the medical field. For this reason, the main focus of this study was to prepare patterned scaffolds using Titanium (Ti) and polyvinyl chloride (PVC) covered on microscope lamellas and examine their liability for cell growth. A polydimethylsiloxane stamp was initially prepared which was then used to transfer a predefined pattern onto PVC- and Ti-covered surfaces. Cell growth experiments were performed on the prepared materials by seeding L929 mouse fibroblasts. The growth of cells seeded on the surface of the scaffolds were spectroscopically followed using Neutral Red uptake assay. The results showed cell proliferation on both patterned surfaces, however, it was higher on Ti-covered samples. In addition, three different alkanethiols were tested for cell adhesion on patterned surfaces. A higher number of cell proliferation was observed with undecanethiol, which has a shorter alkane group among them. The morphological properties of the samples before and after cell-seeding were analyzed via scanning electron microscope and optical microscopy. Significant amount of cell proliferation was observed on all of the prepared samples.

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Acknowledgements

This work was supported by the Research Fund of the Istanbul University-Cerrahpasa. Project Number: BAP-42735. Financial support of Istanbul University—Cerrahpasa is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Gebze Technical University, 41400, Gebze, Kocaeli, Turkey

    Hidayet Sariogullari

  2. Department of Chemistry, Engineering Faculty, Istanbul University-Cerrahpasa, Avcilar, 34320, Istanbul, Turkey

    Ayse Z. Aroguz

  3. Basic Medical Sciences, Department of Molecular Biology and Genetics, Koc University, Istanbul, Turkey

    Zelal Adiguzel

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  1. Hidayet Sariogullari
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  3. Zelal Adiguzel
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All authors contributed to the study conception and design, material preparation, data collection and analysis. The first draft of the manuscript was written by AZA and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ayse Z. Aroguz.

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Sariogullari, H., Aroguz, A.Z. & Adiguzel, Z. Fabrication of a Patterned Scaffold Using Soft Lithography Technique to be Used in Cell Growth Applications. Mol Biotechnol 65, 786–793 (2023). https://doi.org/10.1007/s12033-022-00581-2

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