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CircRNA GRB10 is a Novel Biomarker for the Accurate Diagnosis of Lumbar Degenerative Disc Disease

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Abstract

Lumbar degenerative disc disease (LDDD) is frequently misdiagnosed as other spine conditions, and the accurate diagnosis is challenging. This study was conducted to explore the role of circRNA GRB10 in the accurate diagnosis of LDDD. This study included 60 cases of LDDD, 60 cases of patients with sacroiliac joint pain (SJP), 60 cases of lumbar disc herniation (LDH), 60 cases of piriformis syndrome (PS), 60 cases of entrapment neuropathy (EN) and 60 cases of healthy controls (HCs). Plasma was obtained from each patient before and after treatment. Expression of GRB10 was studied with RT-qPCR. The role of plasma GRB10 in the accurate diagnosis of LDDD was analyzed by ROC curve analysis. Compared to HCs, decreased accumulation of GRB10 RNA was only observed in LDDD group, but not in SJP, LDH, PS and EN groups. With plasma expression level of GRB10 measured before treatment as a biomarker, LDDD patients were separated from SJP, LDH, PS, EN and HC groups. After treatment, increased expression levels of GRB10 were only observed in LDDD group, but not in other groups. Therefore, GRB10 was downregulated in LDDD and may serve as a biomarker for the accurate diagnosis of LDDD.

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Data Availability

The data were available from corresponding author upon reasonable request.

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Acknowledgements

Not applicable.

Funding

This work is supported by Science and Technology Innovation Action Plan of Shanghai Medical Innovation Research Project (Grant Number 20Y11913000); Sailing Plan of Shanghai Science and Technology Committee (Grant Number 21YF1439000).

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Author notes

  1. Licheng Wei and Ji Guo have contributed equally to this work.

Authors and Affiliations

  1. Guanghua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200052, People’s Republic of China

    Licheng Wei, Ji Guo, Weifeng Zhai, Yue Xie & Yongwei Jia

  2. Shanghai Guanghua Hospital of Integrative Medicine, Shanghai, 200052, People’s Republic of China

    Licheng Wei, Ji Guo, Weifeng Zhai, Yue Xie & Yongwei Jia

  3. Institute of Arthritis Research in Integrative Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai, 200052, People’s Republic of China

    Licheng Wei, Ji Guo, Weifeng Zhai, Yue Xie & Yongwei Jia

  4. Department of Spinal Surgery, Shanghai Guanghua Hospital of Integrative Medicine, NO.540, Xinhua Road, Changning District, Shanghai, 200052, People’s Republic of China

    Yongwei Jia

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  1. Licheng Wei
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  2. Ji Guo
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Contributions

Conception and design: YJ. Administrative support: All authors. Provision of study materials or patients: All authors. Collection and assembly of data: All authors. Data analysis and interpretation: All authors. Manuscript writing: LW, JG. Final approval of manuscript: All authors.

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Correspondence to Yongwei Jia.

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The author reports no conflict of interest in this work.

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The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. For human experiments, the trial was conducted in accordance with the Declaration of Helsinki (as revised in 2013). The study was approved by the human Ethics Committee of the Shanghai Guanghua Hospital of Integrative Medicine and informed consent was taken from all individual participants.

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Wei, L., Guo, J., Zhai, W. et al. CircRNA GRB10 is a Novel Biomarker for the Accurate Diagnosis of Lumbar Degenerative Disc Disease. Mol Biotechnol 65, 816–821 (2023). https://doi.org/10.1007/s12033-022-00574-1

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  • DOI: https://doi.org/10.1007/s12033-022-00574-1

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