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Identification and Functional Analysis of the Promoter of a Leucoanthocyanidin Reductase Gene from Gossypium hirsutum

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Abstract

Leucoanthocyanidin reductase (LAR) is the critical enzyme in the synthesis pathway of proanthocyanidins, which are the primary pigments in brown cotton fibers. Our previous study has revealed significant differences in the expression levels of GhLAR1 between white and brown cotton fibers at 10 DPA. In this work, the expression pattern of the GhLAR1 gene was further studied, and the promoter of GhLAR1 (1780 bp) was isolated and characterized. Bioinformatic analysis indicated that GhLAR1 promoter contained many known light response elements and several defenses related to transcriptional factor-binding boxes, which may partially explain the response of the GhLAR1 to temperature, NaCl, and PEG treatments. Furthermore, GhLAR1 was preferentially and strongly expressed in fibers and flowers of cotton, and the expression levels in all tested tissues (especially fibers) of brown cotton were significantly higher than those in white cotton. Consistent with the expression analysis, the GhLAR1 promoter mainly drove GUS expression in epidermal trichomes and floral organs.

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Funding

This work was supported by the Natural Science Foundation of Zhejiang Province, China (LQ22C150004) and the Fundamental Research Funds of Zhejiang Sci-Tech University (11613132612007). We thank all colleagues for their constructive discussions and technical supports.

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Authors and Affiliations

  1. Plant Genomics and Molecular Improvement of Colored Fiber Laboratory, Zhejiang Sci-Tech University, Hangzhou, 310018, China

    Xiaoli Wang, Bo Yuan, Ning Zhu, Rongrong Mu, Hongli Zheng, Yanyan Zhao, Jun Mei, Dongliang Yu, Liping Ke, Yuqiang Sun & Cai Fangfang

  2. Hangzhou Vocational & Technical College, Hangzhou, 310018, China

    Changsheng Shao

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  1. Xiaoli Wang
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  2. Bo Yuan
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Wang, X., Yuan, B., Zhu, N. et al. Identification and Functional Analysis of the Promoter of a Leucoanthocyanidin Reductase Gene from Gossypium hirsutum. Mol Biotechnol 65, 645–654 (2023). https://doi.org/10.1007/s12033-022-00571-4

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