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LncRNA SNHG16 is Downregulated in Pneumonia and Downregulates miR-210 to Promote LPS-Induced Lung Cell Apoptosis

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Abstract

Long non-coding RNA Small Nucleolar RNA Host Gene 16 (SNHG16) has been reported to participate in Lipopolysaccharide (LPS)-induced inflammatory pathway, which contributes to pneumonia. This study was therefore conducted to explore the role of SNHG16 in pneumonia. In this study, expression of SNHG16 and microRNA (miR)-210 in pneumonia plasma samples (n = 56) and control samples (n = 60) was detected by RT-qPCR. The potential crosstalk between SNHG16 and miR-210 was analyzed by performing overexpression experiments. MSP was performed to study the role of SNHG16 in methylation of miR-210 gene. Cell apoptosis was analyzed by cell apoptosis assay. Decreased expression levels of SNHG16 and increased expression levels of miR-210 were observed in pneumonia. SNHG16 showed an inverse correlation to miR-210. LPS treatment led to downregulated SNHG16 and upregulated miR-210 in Human Bronchial Epithelial Cells (HBEpCs). In HBEpCs, SNHG16 downregulated miR-210 and increased miR-210 DNA gene methylation. Moreover, SNHG16 suppressed the role of miR-210 in cell apoptosis under LPS treatment. In conclusion, SNHG16 is downregulated in pneumonia, and it downregulates miR-210 possibly through methylation to promote lung cell apoptosis induced by LPS.

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Authors and Affiliations

  1. Xinjiang Laboratory of Respiratory Disease Research, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University, No. 116 Huanghe Road, Urumqi, 830011, Xinjiang, People’s Republic of China

    Panjun Gao, Jing Wang, Ming Jiang, Zheng Li, Dan Xu, Jing Jing,  Yihepaer & Tingting Hu

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  1. Panjun Gao
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  2. Jing Wang
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  3. Ming Jiang
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  4. Zheng Li
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  6. Jing Jing
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Correspondence to Jing Wang.

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12033_2022_545_MOESM1_ESM.tif

Supplementary file1 The role of overexpression of SNHG16 and miR-210 in regulating the expression of SNHG16 and miR-210 in medium. Culture medium of HBEpCs was collected at 48-h post-transfection to detect circulating miR-210 (A) and SNHG16 (B). *, p < 0.05 (TIF 92 KB)

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Gao, P., Wang, J., Jiang, M. et al. LncRNA SNHG16 is Downregulated in Pneumonia and Downregulates miR-210 to Promote LPS-Induced Lung Cell Apoptosis. Mol Biotechnol 65, 446–452 (2023). https://doi.org/10.1007/s12033-022-00545-6

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  • DOI: https://doi.org/10.1007/s12033-022-00545-6

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