Abstract
In this study, we sought to investigate the prospective role of circ 001372 in modifying inflammation in ovalbumin-induced asthma. In the vivo model of asthma, the serum of circ 001372 was reduced. Down-regulation of circ 001372 increased inflammation reaction (TNF-α, IL-1β, IL-6, and IL-18) and induced COX-2 and iNOS protein expression in vitro model through activation of NFAT5 and suppression of Sirt1. Up-regulation of circ 001372 decreased inflammation reaction (TNF-α, IL-1β, IL-6, and IL-18) in vitro model through inactivation of NFAT5 and induction of Sirt1 by miRNA-128-3p. The miRNA-128-3p lowered the effects of circ 001372 on inflammation in vitro model. The Sirt1 inhibitor reduced the effects of circ 001372 on inflammation in vitro model. Our results revealed the serum of circ 001372 against inflammation in ovalbumin-induced asthma through Sirt1/NFAT5 by miRNA-128-3p.
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Abbreviations
- NFAT:
-
Nuclear factor of activated T cells
- CaN:
-
Calcineurin
- HE:
-
Hematoxylin–eosin
- DMEM:
-
Dulbecco’s modified Eagle’s medium
- FBS:
-
Fetal bovine serum
- TBST:
-
Tris buffer solution tween
- ECL:
-
Electro-chemi-luminescence
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HRL is responsible to the guarantor of integrity of the entire study, study concepts and design, definition of intellectual content, literature research, clinical studies, experimental studies, data acquisition and analysis, statistical analysis, and manuscript preparation and editing; NJW is responsible to the manuscript review. All authors are approved to this manuscript.
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This experiment was approved by the Ethics Committee of Beijing Jishuitan Hospital. BABL/c mice were were obtained from the Laboratory Animal Center of Beijing University. BABL/c mice were obtained from the our hospital.
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Lin, H., Wan, N. Circular RNA has Circ 001372-Reduced Inflammation in Ovalbumin-Induced Asthma Through Sirt1/NFAT5 Signaling Pathway by miRNA-128-3p. Mol Biotechnol 64, 1034–1044 (2022). https://doi.org/10.1007/s12033-022-00480-6
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DOI: https://doi.org/10.1007/s12033-022-00480-6