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The Highly Expressed IFIT1 in Nasopharyngeal Carcinoma Enhances Proliferation, Migration, and Invasion of Nasopharyngeal Carcinoma Cells

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Abstract

In this study, we aimed to identify potential targets modulating the progression of nasopharyngeal carcinoma (NPC) using integrated bioinformatics analysis and functional assays. Differentially expressed genes (DEGs) between NPC and normal tissues samples were obtained from publicly availably microarray datasets (GSE68799, GSE34573, and GSE53819) in the Gene Expression Omnibus (GEO) database. The bioinformatics analysis identified 49 common DEGs from three GEO datasets, which were mainly enriched in cytokine/chemokine pathways and extracellular matrix organization pathway. Further protein–protein interaction network analysis identified 11 hub genes from the 49 DEGs. The 11 hub genes were significantly up-regulated in the NPC tissues when compared to normal tissues by analyzing the Oncomine database. The 8 hub genes including COL5A1, COL7A1, COL22A1, CXCL11, IFI44L, IFIT1, RSAD2, and USP18 were significantly up-regulated in the NPC tissues when compared to normal tissues by using the Oncomine database. Further validation studies showed that IFIT1 was up-regulated in the NPC cells. Knockdown of IFI1T1 suppressed the proliferation, migration, and invasion of NPC cells; while IFIT1 overexpression promoted the proliferation, migration, and invasion of NPC cells. In conclusion, a total of 49 DEGs and 11 hub genes in NPC using the integrated bioinformatics analysis. IFIT1 was up-regulated in the NPC cells lines, and IFIT1 may act as an oncogene by promoting NPC cell proliferation, migration, and invasion.

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Funding

Natural Science Foundation of Guangdong Grants (Nos. 2016A030313381, 2016A030310242); The National Natural Science Foundation Project of China (No. 81602489); Shenzhen Sanming Project (No. SZSM201612041); Shenzhen Science and Technology Innovation Commission Project (Nos. GJHZ20180420180754917, ZDSYS20190902092855097).

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  1. Xuan Wu, Liping Lin, and Fengrui Zhou have equally contributed to this work.

Authors and Affiliations

  1. Department of Oncology, Peking University Shenzhen Hospital, Shenzhen, 518036, China

    Xuan Wu, Fengrui Zhou, Shaokang Yu & Shubin Wang

  2. Shenzhen Key Laboratory of Gastrointestinal Cancer Translational Research, Shenzhen, 518036, China

    Xuan Wu, Fengrui Zhou, Shaokang Yu & Shubin Wang

  3. Cancer Institute of Shenzhen-PKU-HKUST Medical Center, Shenzhen, 518036, China

    Xuan Wu, Fengrui Zhou, Shaokang Yu & Shubin Wang

  4. Department of Oncology, Guangzhou Panyu Central Hospital, Guangzhou, 511400, China

    Liping Lin

  5. Community Healthcare Center, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, 518033, China

    Minhua Chen

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Wu, X., Lin, L., Zhou, F. et al. The Highly Expressed IFIT1 in Nasopharyngeal Carcinoma Enhances Proliferation, Migration, and Invasion of Nasopharyngeal Carcinoma Cells. Mol Biotechnol 64, 621–636 (2022). https://doi.org/10.1007/s12033-021-00439-z

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