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Long Noncoding RNA CRNDE Functions as an Oncogene to Facilitate Aggressive Behaviors of Nasopharyngeal Carcinoma Cells by Modulating miR-3163/TWIST1 Axis

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Abstract

Long noncoding RNA (lncRNA) CRNDE has been broadly implicated in many malignancies. The aim of this study was to explore the function and potential mechanisms of CRNDE in nasopharyngeal carcinoma (NPC). Here, we discovered that CRNDE level was increased in NPC tissues and cell lines. Additionally, elevated CRNDE positively correlated with large tumor size, advanced TNM stage, distant metastasis, EBV infection and worse prognosis. Furthermore, depletion of CRNDE significantly impaired the capacity of proliferation, migration and invasion in NPC cells. Mechanically, CRNDE could serve as a molecular sponge of miR-3163 to regulate the expression of TWIST1. Importantly, the inhibitory effects of CRNDE knockdown on cell proliferation and metastasis were blocked by silence of miR-3163 or restoration of TWIST1 expression. Overall, our data highlighted that CRNDE could promote NPC progression via altering miR-3163/TWIST1 axis, suggesting CRNDE as a potential prognostic biomarker and therapeutic target for NPC treatment.

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Authors and Affiliations

  1. Department of Otolaryngology Head & Neck Surgery, School of Medicine, Shanghai Ninth People’s Hospital, Shanghai JiaoTong University, Shanghai, 200011, China

    Yiyuan Sun, Liuqing Zhang, Qingwei Wu, Chenjie Xu & Peihua Wang

  2. School of Medicine, Ear Institute, Shanghai JiaoTong University, Shanghai, 200011, China

    Yiyuan Sun, Liuqing Zhang, Qingwei Wu, Chenjie Xu & Peihua Wang

  3. Shanghai Key Laboratory of Translational Medicine On Ear and Nose Diseases, Shanghai, 200011, China

    Yiyuan Sun, Liuqing Zhang, Qingwei Wu, Chenjie Xu & Peihua Wang

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  1. Yiyuan Sun
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  2. Liuqing Zhang
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  3. Qingwei Wu
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  4. Chenjie Xu
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  5. Peihua Wang
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Correspondence to Peihua Wang.

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Sun, Y., Zhang, L., Wu, Q. et al. Long Noncoding RNA CRNDE Functions as an Oncogene to Facilitate Aggressive Behaviors of Nasopharyngeal Carcinoma Cells by Modulating miR-3163/TWIST1 Axis. Mol Biotechnol 64, 463–471 (2022). https://doi.org/10.1007/s12033-021-00425-5

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